ArticleMolecular biology of the cell1993
Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.
Article in Molecular biology of the cell, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 74 citations in OpenAlex.
- Non-muscle myosin 2 at a glance.Journal of cell science · 2023Review
- Endogenous species of mammalian nonmuscle myosin IIA and IIB include activated monomers and heteropolymers.Current biology : CB · 2014Article
- Spontaneous contractility-mediated cortical flow generates cell migration in three-dimensional environments.Biophysical journal · 2011Article
- Protrusion and actin assembly are coupled to the organization of lamellar contractile structures.Experimental cell research · 2010Article
- A numerical model of cellular blebbing: a volume-conserving, fluid-structure interaction model of the entire cell.Journal of biomechanics · 2010Article
- Cytoskeleton reorganization in influenza hemagglutinin-initiated syncytium formation.Biochimica et biophysica acta · 2009Article
- Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells.Molecular biology of the cell · 2009Article
- Identification and characterization of Myosin from rat testicular peritubular myoid cells.Biology of reproduction · 2008Article
- The C-terminal tail region of nonmuscle myosin II directs isoform-specific distribution in migrating cells.Molecular biology of the cell · 2008Article
- The forces behind cell movement.International journal of biological sciences · 2007Review
- The role of myosin II motor activity in distributing myosin asymmetrically and coupling protrusive activity to cell translocation.Molecular biology of the cell · 2006Article
- Simultaneous stretching and contraction of stress fibers in vivo.Molecular biology of the cell · 2004Article
- Asymmetric distribution of myosin IIB in migrating endothelial cells is regulated by a rho-dependent kinase and contributes to tail retraction.Molecular biology of the cell · 2003Article
- Keratocytes generate traction forces in two phases.Molecular biology of the cell · 1999Article
- Regulation of cell contraction and membrane ruffling by distinct signals in migratory cells.The Journal of cell biology · 1999Article
- Myosin light chain kinase functions downstream of Ras/ERK to promote migration of urokinase-type plasminogen activator-stimulated cells in an integrin-selective manner.The Journal of cell biology · 1999Article
- Analysis of the actin-myosin II system in fish epidermal keratocytes: mechanism of cell body translocation.The Journal of cell biology · 1997Article
- Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells.Molecular biology of the cell · 1997Article
- Regulation of cell motility by mitogen-activated protein kinase.The Journal of cell biology · 1997Article
- Differential localization of myosin and myosin phosphatase subunits in smooth muscle cells and migrating fibroblasts.Molecular biology of the cell · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Assembly and motor activity of myosin II affect shape, contractility, and locomotion of nonmuscle cells. We used fluorescent analogues and imaging techniques to elucidate the state of assembly and three-dimensional distribution of myosin II in living Swiss 3T3 fibroblasts. An analogue of myosin II that was covalently cross-linked in the 10S conformation and unable to assemble served as an indicator of the cytoplasmic volume accessible to 10S myosin II. Ratio-imaging of an analogue that can undergo 10S-->6S conversion versus the volume indicator revealed localized concentration of assembly-competent myosin II. In stationary serum-deprived cells and in cells locomoting at the edge of a wound, it was most concentrated in the peripheral cytoplasm, where fibers containing myosin II assemble, and least concentrated in the perinuclear cytoplasm, where they disassemble. Furthermore, fluorescence photobleaching recovery showed myosin II to be less mobile in the periphery than in perinuclear cytoplasm. These results indicate a gradient in the assembly of myosin II. Three-dimensional microscopy of living cells revealed that fibers containing myosin II were localized in the cortical cytoplasm, whereas myosin II was diffusely distributed in the deeper cytoplasm, suggesting that myosin II is assembled preferentially near the cell surface. Localized protein phosphorylation may play a role, because a kinase inhibitor, staurosporine, abolished the gradient of myosin II assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.