ArticleBiophysical journal1993
Slow and incomplete inactivations of voltage-gated channels dominate encoding in synthetic neurons.
Article in Biophysical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Conformational Dynamic Studies of Prokaryotic Potassium Channels Explored by Homo-FRET Methodologies.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.International journal of molecular sciences · 2020Review
- Optically activated, customizable, excitable cells.PloS one · 2020Article
- Article
- Endogenous Bioelectric Signaling Networks: Exploiting Voltage Gradients for Control of Growth and Form.Annual review of biomedical engineering · 2017Review
- Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels.eLife · 2016Article
- Engineering biosynthetic excitable tissues from unexcitable cells for electrophysiological and cell therapy studies.Nature communications · 2011Article
- Lessons from models of pancreatic beta cells for engineering glucose-sensing cells.Mathematical biosciences · 2010Review
- Inverse coupling in leak and voltage-activated K+ channel gates underlies distinct roles in electrical signaling.Nature structural & molecular biology · 2009Article
- Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system.Journal of virology · 2000Article
- Contribution of the selectivity filter to inactivation in potassium channels.Biophysical journal · 1999Article
- Modulation of C-type inactivation by K+ at the potassium channel selectivity filter.Biophysical journal · 1998Article
- Interaction between fast and slow inactivation in Skm1 sodium channels.Biophysical journal · 1996Article
- Kinetic characterization of rat brain type IIA sodium channel alpha-subunit stably expressed in a somatic cell line.The Journal of physiology · 1995Article
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Authors and funding
9 authors.
Funding
Abstract
Electrically excitable channels were expressed in Chinese hamster ovary cells using a vaccinia virus vector system. In cells expressing rat brain IIA Na+ channels only, brief pulses (< 1 ms) of depolarizing current resulted in action potentials with a prolonged (0.5-3 s) depolarizing plateau; this plateau was caused by slow and incomplete Na+ channel inactivation. In cells expressing both Na+ and Drosophila Shaker H4 transient K+ channels, there were neuron-like action potentials. In cells with appropriate Na+/K+ current ratios, maintaining stimulation produced repetitive firing over a 10-fold range of frequencies but eventually led to "lock-up" of the potential at a positive value after several seconds of stimulation. The latter effect was due primarily to slow inactivation of the K+ currents. Numerical simulations of modified Hodgkin-Huxley equations describing these currents, using parameters from voltage-clamp kinetics studied in the same cells, accounted for most features of the voltage trajectories. The present study shows that insights into the mechanisms for generating action potentials and trains of action potentials in real excitable cells can be obtained from the analysis of synthetic excitable cells that express a controlled repertoire of ion channels.
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