ArticleThe Biochemical journal1993
Characterization of glycoinositol phospholipids in the amastigote stage of the protozoan parasite Leishmania major.
Article in The Biochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Free GPIs and Comparison of GPI Structures Among Species.International journal of molecular sciences · 2025Review
- Mutations Inactivating Biosynthesis of Dispensable Carbohydrate-Antigens Prevented Extinctions in Primate/Human Lineage Evolution.Journal of molecular evolution · 2025Review
- The Glycosylphosphatidylinositol Anchor: A Linchpin for Cell Surface Versatility of Trypanosomatids.Frontiers in cell and developmental biology · 2021Review
- Extracellular Vesicles in Trypanosomatids: Host Cell Communication.Frontiers in cellular and infection microbiology · 2020Review
- An α-Gal-containing neoglycoprotein-based vaccine partially protects against murine cutaneous leishmaniasis caused by Leishmania major.PLoS neglected tropical diseases · 2017Article
- Leishmania parasites possess a platelet-activating factor acetylhydrolase important for virulence.Molecular and biochemical parasitology · 2012Article
- Endocytosis and Sphingolipid Scavenging in Leishmania mexicana Amastigotes.Biochemistry research international · 2012Article
- Sphingolipids in parasitic protozoa.Advances in experimental medicine and biology · 2010Review
- Toll-like receptors and leishmaniasis.Infection and immunity · 2008Review
- Redirection of sphingolipid metabolism toward de novo synthesis of ethanolamine in Leishmania.The EMBO journal · 2007Article
- Leishmania salvage and remodelling of host sphingolipids in amastigote survival and acidocalcisome biogenesis.Molecular microbiology · 2005Article
- Role of beta-D-galactofuranose in Leishmania major macrophage invasion.Infection and immunity · 2002Article
- Secretory pathway of trypanosomatid parasites.Microbiology and molecular biology reviews : MMBR · 2002Review
- Proteophosphoglycans of Leishmania mexicana. Identification, purification, structural and ultrastructural characterization of the secreted promastigote proteophosphoglycan pPPG2, a stage-specific glycoisoform of amastigote aPPG.The Biochemical journal · 1999Article
- The surface glycoconjugates of trypanosomatid parasites.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1997Review
- Early steps in glycosylphosphatidylinositol biosynthesis in Leishmania major.The Biochemical journal · 1997Article
- Structural studies on a lipoarabinogalactan of Crithidia fasciculata.The Biochemical journal · 1996Article
- The glycoinositol-phospholipids of Phytomonas.The Biochemical journal · 1995Article
- Protein trafficking in kinetoplastid protozoa.Microbiological reviews · 1995Review
- Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major.The Biochemical journal · 1995Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The major macromolecules on the surface of the parasitic protozoan Leishmania major appear to be down-regulated during transformation of the parasite from an insect-dwelling promastigote stage to an intracellular amastigote stage that invades mammalian macrophages. In contrast, the major parasite glycolipids, the glycoinositol phospholipids (GIPLs), are shown here to be expressed at near-constant levels in both developmental stages. The structures of the GIPLs from tissue-derived amastigotes have been determined by h.p.l.c. analysis of the deaminated and reduced glycan head groups, and by chemical and enzymic sequencing. The deduced structures appear to form a complete biosynthetic series, ranging from Man alpha 1-4GlcN-phosphatidylinositol (PI) to Gal alpha 1-3Galf beta 1-3Man alpha 1-3Man alpha 1-4GlcN-PI (GIPL-2). A small proportion of GIPL-2 was further extended by addition of a Gal residue in either alpha 1-6 or beta 1-3 linkage. From g.c.-m.s. analysis and mild base treatment, all the GIPLs were shown to contain either alkylacylglycerol or lyso-alkylglycerol lipid moieties, where the alkyl chains were predominantly C18:0, with lower levels of C20:0, C22:0 and C24:0. L. major amastigotes also contained at least two PI-specific phospholipase C-resistant glycolipids which are absent from promastigotes. These neutral glycolipids were resistant to both mild acid and mild base hydrolysis, contained terminal beta-Gal residues and were not lost during extensive purification of amastigotes from host cell membranes. It is likely that these glycolipids are glycosphingolipids acquired from the mammalian host. The GIPL profile of L. major amastigotes is compared with the profiles found in L. major promastigotes and L. donovani amastigotes.
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