ArticleProceedings of the National Academy of Sciences of the United States of America1993
Analysis of somatic hypermutation in mouse Peyer's patches using immunoglobulin kappa light-chain transgenes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 106 citations in OpenAlex.
- Muons, mutations, and planetary shielding.Frontiers in astronomy and space sciences · 2022Article
- Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches.Journal of visualized experiments : JoVE · 2021Article
- Article
- p21 is dispensable for AID-mediated class switch recombination and mutagenesis of immunoglobulin genes during somatic hypermutation.Molecular immunology · 2011Article
- Class switch recombination and somatic hypermutation in early mouse B cells are mediated by B cell and Toll-like receptors.Immunity · 2007Article
- Negative regulation of activation-induced cytidine deaminase in B cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The translesion DNA polymerase theta plays a dominant role in immunoglobulin gene somatic hypermutation.The EMBO journal · 2005Article
- Expression of human AID in yeast induces mutations in context similar to the context of somatic hypermutation at G-C pairs in immunoglobulin genes.BMC immunology · 2005Article
- Study of humoral immunity to commensal oral bacteria in human infants demonstrates the presence of secretory immunoglobulin A antibodies reactive with Actinomyces naeslundii genospecies 1 and 2 ribotypes.Clinical and diagnostic laboratory immunology · 2004Article
- Correlation of somatic hypermutation specificity and A-T base pair substitution errors by DNA polymerase eta during copying of a mouse immunoglobulin kappa light chain transgene.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The intrinsic hypermutability of antibody heavy and light chain genes decays exponentially.The EMBO journal · 2001Article
- Article
- Both DNA strands of antibody genes are hypermutation targets.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Automated detection of point mutations using fluorescent sequence trace subtraction.Nucleic acids research · 1998Article
- Altered spectra of hypermutation in antibodies from mice deficient for the DNA mismatch repair protein PMS2.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Low antigen dose favours selection of somatic mutants with hallmarks of antibody affinity maturation.Immunology · 1998Article
- Inherent properties of somatic hypermutation as revealed by human non-productive VH6 immunoglobulin rearrangements.Immunology · 1998Article
- Cells strongly expressing Ig(kappa) transgenes show clonal recruitment of hypermutation: a role for both MAR and the enhancers.The EMBO journal · 1997Article
- Rapid methods for the analysis of immunoglobulin gene hypermutation: application to transgenic and gene targeted mice.Nucleic acids research · 1997Article
- Modifying the sequence of an immunoglobulin V-gene alters the resulting pattern of hypermutation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have exploited mice transgenic for an immunoglobulin kappa light chain in order to show that immunoglobulin genes in the B cells of Peyer's patches in unimmunized mice carry a high level of somatic mutations. Most of the mutations are found in the subpopulation of B cells which, based on peanut agglutinin binding, derive from the germinal centers. The number of mutations per clone and their distribution along the variable gene segment (indicative of untemplated point mutations) are very similar to those found in antigen-specific splenic B cells of normal mice after secondary immunization. The mutations accumulate mainly in complementarity-determining region 1, in particular in some specific codons (Ser-26, Ser-31, and Ser-77) which have been previously recognized as intrinsic hypermutational hotspots. These results suggest that, as in the spleen, somatic mutation occurs in B cells which have migrated to the germinal centers, probably as a consequence of stimulation by antigens present in the gut environment. Transgenic animals are increasingly being used to define the signals involved in hypermutation. However, their subsequent study is very time-consuming because it is based on immunization and analysis of hybridomas or antigen-selected cells. We propose that the use of Peyer's patches of unimmunized adult mice offers a reliable and simple approach to analyze hypermutation of transgenes.
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