ArticleProceedings of the National Academy of Sciences of the United States of America1993
Differential expression of four members of the POU family of proteins in activated and phorbol 12-myristate 13-acetate-treated Jurkat T cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Vascular dysfunction caused by loss of Brn-3b/POU4F2 transcription factor in aortic vascular smooth muscle cells is linked to deregulation of calcium signalling pathways.Cell death & disease · 2023Article
- Characterization of the HHV-6B U20 Immunoevasin.Journal of virology · 2023Article
- Linking metabolic dysfunction with cardiovascular diseases: Brn-3b/POU4F2 transcription factor in cardiometabolic tissues in health and disease.Cell death & disease · 2021Review
- Essential but partially redundant roles for POU4F1/Brn-3a and POU4F2/Brn-3b transcription factors in the developing heart.Cell death & disease · 2017Article
- Octamer-dependent transcription in T cells is mediated by NFAT and NF-κB.Nucleic acids research · 2013Article
- Brn-3a neuronal transcription factor functional expression in human prostate cancer.Prostate cancer and prostatic diseases · 2006Article
- Stimulation-induced gene expression in Ramos B-cells.Genes and immunity · 2003Article
- Jun kinase phosphorylates and regulates the DNA binding activity of an octamer binding protein, T-cell factor beta1.Molecular and cellular biology · 1999Article
- Oct-1 [corrected] and Oct-2 DNA-binding site specificity is regulated in vitro by different kinases.The Biochemical journal · 1996Article
- Multiplex selection technique (MuST): an approach to clone transcription factor binding sites.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Analysis of differential gene expression by display of 3' end restriction fragments of cDNAs.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The POU family of proteins binds specifically to octamer DNA motifs present in the promoters of several genes and regulates their expression. We identified the presence of four members of the POU family of proteins, Oct-1, Oct-2, Oct-T1, and Oct-T2, in the human T-cell line Jurkat. To obtain insight into the physiological role played by these proteins in T cells, we studied the time course of expression of these genes in resting, activated, and phorbol 12-myristate 13-acetate (PMA)-treated cells. The expression of the gene encoding Oct-1 (now assigned the name OTF1 for octamer-binding transcription factor 1) remained unchanged and the levels of Oct-T2 mRNA decreased with increasing time of incubation to undetectable amounts in all three states of T-cell growth. The levels of Oct-2 mRNA and protein were increased in activated cells, were increased to a lesser extent in the PMA-treated cells, and were undetectable in resting cells. The levels of the Oct-T1 transcripts increased dramatically in PMA-treated cells but not in resting or activated cells. Sequence analysis of the Oct-T1 cDNA showed an open reading frame coding for a POU domain-containing protein of 42.7 kDa. Transient transfection of the gene encoding Oct-T1 decreased the activity of the interleukin 2 gene promoter in activated Jurkat cells. Further, there is evidence for an additional octamer-binding protein, Oct-T3, in Jurkat T cells.
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