ArticleProceedings of the National Academy of Sciences of the United States of America1993
Determination of mRNA fate by different RNA polymerase II promoters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- The circular logic of mRNA homeostasis.Transcription · 2023Review
- Global translation inhibition yields condition-dependent de-repression of ribosome biogenesis mRNAs.Nucleic acids research · 2019Article
- Spt6 Association with RNA Polymerase II Directs mRNA Turnover During Transcription.Molecular cell · 2018Article
- Antisense transcription-dependent chromatin signature modulates sense transcript dynamics.Molecular systems biology · 2018Article
- Measuring mRNA Decay in Budding Yeast Using Single Molecule FISH.Methods in molecular biology (Clifton, N.J.) · 2018Article
- The interplay between transcription and mRNA degradation inMicrobial cell (Graz, Austria) · 2017Review
- A novel role for CARM1 in promoting nonsense-mediated mRNA decay: potential implications for spinal muscular atrophy.Nucleic acids research · 2016Article
- Coupling mRNA synthesis and decay.Molecular and cellular biology · 2014Review
- Gtpbp2 is required for BMP signaling and mesoderm patterning in Xenopus embryos.Developmental biology · 2014Article
- A mental retardation-linked nonsense mutation in cereblon is rescued by proteasome inhibition.The Journal of biological chemistry · 2013Article
- mRNA quality control pathways in Saccharomyces cerevisiae.Journal of biosciences · 2013Review
- Temporal and spatial characterization of nonsense-mediated mRNA decay.Genes & development · 2013Article
- Widespread promoter-mediated coordination of transcription and mRNA degradation.Genome biology · 2012Article
- Unspliced precursors of NMD-sensitive β-globin transcripts exhibit decreased steady-state levels in erythroid cells.PloS one · 2012Article
- Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells.Nucleic acids research · 2009Article
- Cell cycle-dependent phosphorylation of human CDC5 regulates RNA processing.Cell cycle (Georgetown, Tex.) · 2008Article
- The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway.Nucleic acids research · 2007Article
- Internal ribosome entry sequence-mediated translation initiation triggers nonsense-mediated decay.EMBO reports · 2006Article
- A 3' UTR sequence stabilizes termination codons in the unspliced RNA of Rous sarcoma virus.RNA (New York, N.Y.) · 2006Article
- Computational definition of sequence motifs governing constitutive exon splicing.Genes & development · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translational stop mutations of the human beta-globin gene cause a reduction of cytoplasmic mRNA accumulation in thalassemia patients and in transfection models. The exact mechanism underlying this phenomenon has remained enigmatic but is known to be post-transcriptional. We have used transfected HeLa cells to study the expression of beta-globin mRNAs with nonsense or frameshift mutations within the three exons of this gene. Mutations in exons 1 or 2 reduce cytoplasmic mRNA accumulation whereas a mutation in exon 3 permits essentially normal expression. We report here that the post-transcriptional fate of mutated beta-globin mRNAs is differentially affected by the type of RNA polymerase II promoter driving expression. Replacement of the beta-globin promoter with the herpes simplex virus type 1 thymidine kinase gene promoter but not the cytomegalovirus immediate early promoter rescues the cytoplasmic accumulation of mutated mRNA to wild-type levels. This effect is shown to be independent of the absolute quantity and the kinetics of accumulation of mutated mRNA synthesized, and primer-extension analyses confirm that both viral promoters accurately utilize identical transcription start sites. These data thus reveal an unexpected property of RNA polymerase II promoters: determination of the post-transcriptional fate of the maturing mRNA, presumably by influencing alternative choices between as yet undefined processing and/or transport pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.