ArticleThe Journal of cell biology1993
Cytoplasmic dynein plays a role in mammalian mitotic spindle formation.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
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Who cites it
143 citing papers in PubMed, 332 citations in OpenAlex.
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- ZW10: an emerging orchestrator of organelle dynamics during the cell division cycle.Journal of molecular cell biology · 2024Review
- Microtubule choreography: spindle self-organization during cell division.Biophysical reviews · 2024Review
- Positioning centrioles and centrosomes.The Journal of cell biology · 2024Review
- Evidence of kinesin motors involved in stable kinetochore assembly during early meiosis.Molecular biology of the cell · 2023Article
- Novel PathogenicJournal of pediatric genetics · 2023Article
- Cortical dynein drives centrosome clustering in cells with centrosome amplification.Molecular biology of the cell · 2023Article
- Interrelated effects of chromosome size, mechanics, number, location-orientation and polar ejection force on the spindle accuracy: a 3D computational study.Molecular biology of the cell · 2023Article
- Aurora A phosphorylates Ndel1 to reduce the levels of Mad1 and NuMA at spindle poles.Molecular biology of the cell · 2023Article
- Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo.Scientific reports · 2022Article
- CellDynaMo-stochastic reaction-diffusion-dynamics model: Application to search-and-capture process of mitotic spindle assembly.PLoS computational biology · 2022Article
- Long-range electrostatic interactions significantly modulate the affinity of dynein for microtubules.Biophysical journal · 2022Article
- Bi-allelic variants in SPATA5L1 lead to intellectual disability, spastic-dystonic cerebral palsy, epilepsy, and hearing loss.American journal of human genetics · 2021Article
- TheCells · 2021Review
- Nano-Particles Carried by Multiple Dynein Motors Self-Regulate Their Number of Actively Participating Motors.International journal of molecular sciences · 2021Article
- Modeling reveals cortical dynein-dependent fluctuations in bipolar spindle length.Biophysical journal · 2021Article
- Mechanisms by Which Kinesin-5 Motors Perform Their Multiple Intracellular Functions.International journal of molecular sciences · 2021Review
- Review: Cytoplasmic dynein motors in photoreceptors.Molecular vision · 2021Review
- Cell polarity-dependent centrosome separation in theThe Journal of cell biology · 2019Article
83 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The formation and functioning of a mitotic spindle depends not only on the assembly/disassembly of microtubules but also on the action of motor enzymes. Cytoplasmic dynein has been localized to spindles, but whether or how it functions in mitotic processes is not yet known. We have cloned and expressed DNA fragments that encode the putative ATP-hydrolytic sites of the cytoplasmic dynein heavy chain from HeLa cells and from Dictyostelium. Monospecific antibodies have been raised to the resulting polypeptides, and these inhibit dynein motor activity in vitro. Their injection into mitotic mammalian cells blocks the formation of spindles in prophase or during recovery from nocodazole treatment at later stages of mitosis. Cells become arrested with unseparated centrosomes and form monopolar spindles. The injected antibodies have no detectable effect on chromosome attachment to a bipolar spindle or on motions during anaphase. These data suggest that cytoplasmic dynein plays a unique and important role in the initial events of bipolar spindle formation, while any later roles that it may play are redundant. Possible mechanisms of dynein's involvement in mitosis are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.