ArticleThe Journal of cell biology1993
Type 2X-myosin heavy chain is coded by a muscle fiber type-specific and developmentally regulated gene.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 221 citations in OpenAlex.
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- Dynamic Changes in the Global Transcriptome of Postnatal Skeletal Muscle in Different Sheep.Genes · 2023Article
- Mitochondrial adaptations to inactivity in diaphragm muscle fibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Article
- Three-dimensional structural analysis of mitochondria composing each subtype of fast-twitch muscle fibers in chicken.The Journal of veterinary medical science · 2022Article
- History and development of staining methods for skeletal muscle fiber types.Histology and histopathology · 2022Review
- Differences in gene expression between the primary and secondary inferior oblique overaction.Translational pediatrics · 2022Article
- Sex differences in metabolic pathways are regulated by Pfkfb3 and Pdk4 expression in rodent muscle.Communications biology · 2021Article
- Advanced Fiber Type-Specific Protein Profiles Derived from Adult Murine Skeletal Muscle.Proteomes · 2021Article
- Effect of high-fat mixed lipid diet and swimming on fibre types in skeletal muscles of rats with colon tumours.European journal of histochemistry : EJH · 2018Article
- Muscle Contraction.Cold Spring Harbor perspectives in biology · 2018Review
- C/EBPα represses slow myosin heavy chain 2 gene expression in developing avian myotubes.Biochimica et biophysica acta · 2016Article
- Effect of altered innervation and thyroid hormones on myosin heavy chain expression and fiber type transitions: a mini-review.Histochemistry and cell biology · 2015Review
- Assessment of muscle mass and strength in mice.BoneKEy reports · 2015Article
- Developmental myosins: expression patterns and functional significance.Skeletal muscle · 2015Article
- A simplified immunohistochemical classification of skeletal muscle fibres in mouse.European journal of histochemistry : EJH · 2014Article
- Single nucleotide polymorphisms, haplotypes and combined genotypes in MYH₃ gene and their associations with growth and carcass traits in Qinchuan cattle.Molecular biology reports · 2013Article
- A novel method to measure glucose uptake and myosin heavy chain isoform expression of single fibers from rat skeletal muscle.Diabetes · 2012Article
- Transcriptomics of a giant freshwater prawn (Macrobrachium rosenbergii): de novo assembly, annotation and marker discovery.PloS one · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously reported the identification of a distinct myosin heavy chain (MyHC) isoform in a major subpopulation of rat skeletal muscle fibers, referred to as 2X fibers (Schiaffino, S., L. Gorza, S. Sartore, L. Saggin, M. Vianello, K. Gundersen, and T. Lømo. 1989. J. Muscle Res. Cell Motil. 10:197-205). However, it was not known whether 2X-MyHC is the product of posttranslational modification of other MyHCs or is coded by a distinct mRNA. We report here the isolation and characterization of cDNAs coding a MyHC isoform that is expressed in type 2X skeletal muscle fibers. 2X-MyHC transcripts differ from other MyHC transcripts in their restriction map and 3' end sequence and are thus derived from a distinct gene. In situ hybridization analyses show that 2X-MyHC transcripts are expressed at high levels in the diaphragm and fast hindlimb muscles and can be coexpressed either with 2B- or 2A-MyHC transcripts in a number of fibers. At the single fiber level the distribution of each MyHC mRNA closely matches that of the corresponding protein, determined by specific antibodies on serial sections. In hindlimb muscles 2X-, 2A-, and 2B-MyHC transcripts are first detected by postnatal day 2-5 and display from the earliest stages a distinct pattern of distribution in different muscles and different fibers. The emergence of type 2 MyHC isoforms thus defines a distinct neonatal phase of fiber type differentiation during muscle development. The functional significance of MyHC isoforms is discussed with particular reference to the velocity of shortening of skeletal muscle fibers.
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