ArticleThe Journal of cell biology1993
The cytoplasmic droplet of rat epididymal spermatozoa contains saccular elements with Golgi characteristics.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 90 citations in OpenAlex.
- Exploring the characters of sperm lipid peroxidation, enzymatic antioxidant activity, and spermatozoa zona binding capacity with aging in dogs.Scientific reports · 2025Article
- TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bovine Spermatogenesis.Advances in anatomy, embryology, and cell biology · 2025Review
- Bull Sperm Abnormalities in Practice.Advances in anatomy, embryology, and cell biology · 2025Review
- SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility.Nature communications · 2023Article
- Immunocytochemical Localization of Olfactory-signaling Molecules in Human and Rat Spermatozoa.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Advances in understanding mechanisms of long-term sperm storage-the soft-shelled turtle model.Histology and histopathology · 2020Article
- Modification of sperm morphology during long-term sperm storage in the reproductive tract of the Chinese soft-shelled turtle, Pelodiscus sinensis.Scientific reports · 2015Article
- Expression, sorting, and segregation of Golgi proteins during germ cell differentiation in the testis.Molecular biology of the cell · 2015Article
- Compartmentalization of membrane trafficking, glucose transport, glycolysis, actin, tubulin and the proteasome in the cytoplasmic droplet/Hermes body of epididymal sperm.Open biology · 2015Article
- The cytoplasmic droplet may be indicative of sperm motility and normal spermiogenesis.Asian journal of andrology · 2013Article
- Proteomic analyses reveal a role of cytoplasmic droplets as an energy source during epididymal sperm maturation.PloS one · 2013Article
- The epididymis, cytoplasmic droplets and male fertility.Asian journal of andrology · 2011Review
- Spam1-associated transmission ratio distortion in mice: elucidating the mechanism.Reproductive biology and endocrinology : RB&E · 2005Article
- Divergent N-terminal sequences target an inducible testis deubiquitinating enzyme to distinct subcellular structures.Molecular and cellular biology · 2000Article
- Complex carbohydrates in drug development.Advances in pharmacology (San Diego, Calif.) · 1992Review
- Alterations in the testis and epididymis associated with loss of function of the cystatin-related epididymal spermatogenic (CRES) protein.Journal of andrologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoplasmic droplet of epididymal spermatozoa is a small localized outpouching of cytoplasm of the tail of unknown significance. EM revealed flattened saccular elements as the near exclusive membranous component of the droplet. Light and electron microscopic immunolabeling for Golgi/TGN markers showed these saccules to be reactive for antibodies to TGN38, protein affinity-purified alpha 2,6 sialyltransferase, and anti-human beta 1,4 galactosyltransferase. The saccules were isolated by subcellular fractionation and antibodies raised against this fraction immunolabeled the saccules of the droplet in situ as well as the Golgi region of somatic epithelial cells lining the epididymis. The isolated droplet fraction was enriched in galactosyltransferase and sialyltransferase activities, and endogenous glycosylation assays identified the modification of several endogenous glycopeptides. EM lectin staining in situ demonstrated galactose and N-acetyl galactosamine constituents in the saccules. Endocytic studies with cationic and anionic ferritin as well as HRP failed to identify the saccules as components of the endocytic apparatus. Epididymal spermatozoa were devoid of markers for the ER as well as the Golgi-associated coatamer protein beta-COP. It is therefore unlikely that the saccular elements of the droplet participate in vesicular protein transport. However, the identification of Golgi/TGN glycosylating activities in the saccules may be related to plasma membrane modifications which occur during epididymal sperm maturation.
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