ArticleThe Journal of cell biology1993
Differential regulation of multiple gap junction transcripts and proteins during rat liver regeneration.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Connexin 32 constrains a mesenchymal-like switch in differentiated urothelium and luminal cancers.Life science alliance · 2026Article
- Biophysical control of plasticity and patterning in regeneration and cancer.Cellular and molecular life sciences : CMLS · 2023Review
- Connexin-based signaling and drug-induced hepatotoxicity.Journal of clinical and translational research · 2017Article
- Roles of connexins and pannexins in digestive homeostasis.Cellular and molecular life sciences : CMLS · 2015Review
- Connexin and pannexin (hemi)channels in the liver.Frontiers in physiology · 2014Review
- Biological role of connexin intercellular channels and hemichannels.Archives of biochemistry and biophysics · 2012Review
- The growth pattern of transplanted normal and nodular hepatocytes.Histochemistry and cell biology · 2011Article
- Polysome trafficking of transcripts and microRNAs in regenerating liver after partial hepatectomy.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Roles of gap junctions and connexins in non-neoplastic pathological processes in which cell proliferation is involved.The Journal of membrane biology · 2007Review
- Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.Molecular biology of the cell · 2006Article
- beta 1C Integrin expression in human endometrial proliferative diseases.The American journal of pathology · 2003Article
- Induction of P-glycoprotein mRNA transcripts by cycloheximide in animal tissues: evidence that class I Pgp is transcriptionally regulated whereas class II Pgp is post-transcriptionally regulated.Molecular and cellular biochemistry · 2001Article
- Bone morphogenetic protein-2 (BMP-2) and transforming growth factor-beta1 (TGF-beta1) alter connexin 43 phosphorylation in MC3T3-E1 Cells.BMC cell biology · 2001Article
- Ursodeoxycholic acid may inhibit deoxycholic acid-induced apoptosis by modulating mitochondrial transmembrane potential and reactive oxygen species production.Molecular medicine (Cambridge, Mass.) · 1998Article
- Connexin46 is retained as monomers in a trans-Golgi compartment of osteoblastic cells.The Journal of cell biology · 1997Article
- mRNA stability in mammalian cells.Microbiological reviews · 1995Review
- Transfected connexin45 alters gap junction permeability in cells expressing endogenous connexin43.The Journal of cell biology · 1995Article
- Single channel behavior of recombinant beta 2 gap junction connexons reconstituted into planar lipid bilayers.Biophysical journal · 1995Article
- Catalase-negative peroxisomes: transient appearance in rat hepatocytes during liver regeneration after partial hepatectomy.The American journal of pathology · 1995Article
- Basal promoter of the rat connexin 32 gene: identification and characterization of an essential element and its DNA-binding protein.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The mRNA and protein expression of alpha 1 (connexin 43), beta 1 (connexin 32), and beta 2 (connexin 26) gap junction genes were examined in the regenerating rat liver after 70% partial hepatectomy (PH). Expression of beta 1 and beta 2 steady-state mRNA levels changed minimally until 12 h after PH when both transcripts decreased to approximately 15% of baseline values. A similar decrease in assembled connexin levels was detected by immunoblot and indirect immunofluorescence at 18 h after PH. Both transcripts simultaneously increased between 24 and 42 h and again rapidly decreased by 48 h post-PH. beta 1 and beta 2 assembled gap junction protein expression increased at 48 h post-PH and rapidly decreased by 56 h. By 72 to 84 h post-PH, beta 1 and beta 2 mRNA and assembled protein expression returned to near baseline levels and were maintained. Interestingly, inhibition of protein synthesis with cycloheximide completely inhibited disappearance of the beta 2 transcript, in contrast to beta 1 mRNA which was unaffected. Nuclear run-on assays showed no change in transcriptional rates for either gene during the regenerative period. However, both beta 1 and beta 2 transcripts exhibited significantly decreased mRNA half-lives at 12 h post-PH (3.8 and 3.7 h, respectively) relative to those at 0 h (10.9 and 6.1 h, respectively). Surprisingly, although the transcriptional rate for alpha 1 was similar to that observed for beta 2, no alpha 1 transcripts were detectable by northern or RNase protection analysis. The results suggest that in the regenerating rat liver, beta 1 and beta 2 gap junction genes are not regulated at the transcriptional level. Rather, the cyclical modulation of their steady-state transcripts is regulated primarily by posttranscriptional events of which mRNA stability is at least one critical factor in the control process.
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