ArticleThe Journal of cell biology1993
Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 101 citations in OpenAlex.
- Structure and Function of p97 and Pex1/6 Type II AAA+ Complexes.Frontiers in molecular biosciences · 2017Review
- Yeast nitrogen utilization in the phyllosphere during plant lifespan under regulation of autophagy.Scientific reports · 2015Article
- The peroxisomal matrix import of Pex8p requires only PTS receptors and Pex14p.Molecular biology of the cell · 2009Article
- Opinion: peroxisomal-protein import: is it really that complex?Nature reviews. Molecular cell biology · 2002Review
- Peroxisomal metabolic function is required for appressorium-mediated plant infection by Colletotrichum lagenarium.The Plant cell · 2001Article
- Direct interaction between glyoxysomes and lipid bodies in cotyledons of the Arabidopsis thaliana ped1 mutant.Protoplasma · 2001Article
- The peroxisome biogenesis factors pex4p, pex22p, pex1p, and pex6p act in the terminal steps of peroxisomal matrix protein import.Molecular and cellular biology · 2000Article
- Peroxisomal membrane fusion requires two AAA family ATPases, Pex1p and Pex6p.The Journal of cell biology · 2000Article
- Fusion of small peroxisomal vesicles in vitro reconstructs an early step in the in vivo multistep peroxisome assembly pathway of Yarrowia lipolytica.The Journal of cell biology · 2000Article
- PEX12 interacts with PEX5 and PEX10 and acts downstream of receptor docking in peroxisomal matrix protein import.The Journal of cell biology · 1999Article
- Pex19p interacts with Pex3p and Pex10p and is essential for peroxisome biogenesis in Pichia pastoris.Molecular biology of the cell · 1999Article
- The surprising complexity of peroxisome biogenesis.Plant molecular biology · 1998Review
- Peroxisome degradation by microautophagy in Pichia pastoris: identification of specific steps and morphological intermediates.The Journal of cell biology · 1998Article
- Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomes.Molecular and cellular biology · 1998Article
- Peroxisomal targeting, import, and assembly of alcohol oxidase in Pichia pastoris.The Journal of cell biology · 1997Article
- The Hansenula polymorpha PEX14 gene encodes a novel peroxisomal membrane protein essential for peroxisome biogenesis.The EMBO journal · 1997Article
- Multiple PEX genes are required for proper subcellular distribution and stability of Pex5p, the PTS1 receptor: evidence that PTS1 protein import is mediated by a cycling receptor.The Journal of cell biology · 1996Article
- Human peroxisome assembly factor-2 (PAF-2): a gene responsible for group C peroxisome biogenesis disorder in humans.American journal of human genetics · 1996Article
- Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTs1 receptor.The Journal of cell biology · 1996Article
- A unified nomenclature for peroxisome biogenesis factors.The Journal of cell biology · 1996Review
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2 authors at 1 institution in 1 country.
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Abstract
The biogenesis and maintenance of cellular organelles is of fundamental importance in all eukaryotic cells. One such organelle is the peroxisome. The establishment of a genetic system to study peroxisome biogenesis in the methylotrophic yeast Pichia pastoris has yielded many different complementation groups of peroxisomal assembly (pas) or peroxisome-deficient (per) mutants. Each appears to be deficient in functional peroxisomes. One of these mutants, pas5, has been characterized, complemented, and the gene sequenced. Ultrastructural studies show that normal peroxisomes are not present in pas5, but aberrant peroxisomal structures resembling "membranous ghosts" are frequently observed. The "peroxisome ghosts" appear to be induced and segregated to daughter cells normally. Biochemical fractionation analysis of organelles of the pas5 mutant reveals that peroxisomal matrix enzymes are induced normally but are found mostly in the cytosol. However, purification of peroxisome ghosts from the mutant shows that small amounts (< 5%) of matrix enzymes are imported. The PAS5 gene was cloned and found to encode a 127-kD protein, which contains a 200-amino acid-long region of homology with PAS1, NEM-sensitive factor (NSF), and other related ATPases. Weak homology to a yeast myosin was also observed. The gene is not essential for growth on glucose but is essential for growth on oleic acid and methanol. The role of PAS5 in peroxisome biogenesis is discussed.
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