ArticleThe Journal of cell biology1993
Membrane-associated lamins in Xenopus egg extracts: identification of two vesicle populations.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 98 citations in OpenAlex.
- Nuclear Lamins: Key Proteins for Embryonic Development.Biology · 2022Review
- Review
- The Dynamics of Lamin a During the Cell Cycle.Frontiers in molecular biosciences · 2021Article
- Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.The Journal of cell biology · 2013Article
- Lamins A and C are present in the nuclei of early porcine embryos, with lamin A being distributed in large intranuclear foci.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Article
- Transmembrane proteins are not required for early stages of nuclear envelope assembly.The Biochemical journal · 2006Article
- Inhibiting farnesylation of progerin prevents the characteristic nuclear blebbing of Hutchinson-Gilford progeria syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.The Journal of cell biology · 2002Article
- The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.The EMBO journal · 2001Article
- A role for nuclear lamins in nuclear envelope assembly.The Journal of cell biology · 2001Article
- Nuclear lamins A and B1: different pathways of assembly during nuclear envelope formation in living cells.The Journal of cell biology · 2000Article
- Association of prenylated proteins with the plasma membrane and the inner nuclear membrane is mediated by the same membrane-targeting motifs.Molecular biology of the cell · 2000Article
- Disruption of nuclear lamin organization blocks the elongation phase of DNA replication.The Journal of cell biology · 2000Article
- Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.The Journal of cell biology · 1999Article
- Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.The Journal of cell biology · 1999Article
- Lamin-binding fragment of LAP2 inhibits increase in nuclear volume during the cell cycle and progression into S phase.The Journal of cell biology · 1997Article
- Nuclear membrane vesicle targeting to chromatin in a Drosophila embryo cell-free system.Molecular biology of the cell · 1997Article
- Localization and posttranslational modifications of otefin, a protein required for vesicle attachment to chromatin, during Drosophila melanogaster development.Molecular and cellular biology · 1997Article
- Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis.The Journal of cell biology · 1997Article
- Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear lamin isoforms of vertebrates can be divided into two major classes. The B-type lamins are membrane associated throughout the cell cycle, whereas A-type lamins are recovered from mitotic cell homogenates in membrane-free fractions. A feature of oogenesis in birds and mammals is the nearly exclusive presence of B-type lamins in oocyte nuclear envelopes. In contrast, oocytes and early cleavage embryos of the amphibian Xenopus laevis are believed to contain a single lamin isoform, lamin LIII, which after nuclear envelope breakdown during meiotic maturation is reported to be completely soluble. Consequently, we have reexamined the lamin complement of Xenopus oocyte nuclear envelopes, egg extracts, and early embryos. An mAb (X223) specific for the homologous B-type lamins B2 of mouse and LII of Xenopus somatic cells (Höger, T., K. Zatloukal, I. Waizenegger, and G. Krohne. 1990. Chromosoma. 99:379-390) recognized a Xenopus oocyte nuclear envelope protein biochemically distinct from lamin LIII and very similar or identical to somatic cell lamin LII. Oocyte lamin LII was detectable in nuclear envelopes of early cleavage embryos. Immunoblotting of fractionated egg extracts revealed that approximately 20-23% of lamin LII and 5-7% of lamin LIII were membrane associated. EM immunolocalization demonstrated that membrane-bound lamins LII and LIII are associated with separate vesicle populations. These findings are relevant to the interpretation of nuclear reconstitution experiments using Xenopus egg extracts.
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