ArticleJournal of bacteriology1993
Hemin uptake in Porphyromonas gingivalis: Omp26 is a hemin-binding surface protein.
Article in Journal of bacteriology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Iron Pathways and Iron Chelation Approaches in Viral, Microbial, and Fungal Infections.Pharmaceuticals (Basel, Switzerland) · 2020Review
- The positive effect of exogenous hemin on a resistance of strict anaerobic archaeon Methanobrevibacter arboriphilus to oxidative stresses.Current microbiology · 2012Article
- Metal uptake in host-pathogen interactions: role of iron in Porphyromonas gingivalis interactions with host organisms.Periodontology 2000 · 2010Review
- LuxS involvement in the regulation of genes coding for hemin and iron acquisition systems in Porphyromonas gingivalis.Infection and immunity · 2006Article
- Identification of amino acid residues involved in heme binding and hemoprotein utilization in the Porphyromonas gingivalis heme receptor HmuR.Infection and immunity · 2006Article
- Porphyrin-mediated cell surface heme capture from hemoglobin by Porphyromonas gingivalis.Journal of bacteriology · 2003Article
- Binding specificity of the Porphyromonas gingivalis heme and hemoglobin receptor HmuR, gingipain K, and gingipain R1 for heme, porphyrins, and metalloporphyrins.Journal of bacteriology · 2001Article
- Degradation of host heme proteins by lysine- and arginine-specific cysteine proteinases (gingipains) of Porphyromonas gingivalis.Journal of bacteriology · 2001Article
- Detection of heme-binding proteins in epidemic strains of Burkholderia cepacia.Clinical and diagnostic laboratory immunology · 2001Article
- Hemin-binding surface protein from Bartonella quintana.Infection and immunity · 2000Article
- Characterization of a novel outer membrane hemin-binding protein of Porphyromonas gingivalis.Journal of bacteriology · 2000Article
- Characterization and expression of HmuR, a TonB-dependent hemoglobin receptor of Porphyromonas gingivalis.Journal of bacteriology · 2000Article
- Hemoglobinase activity of the lysine gingipain protease (Kgp) of Porphyromonas gingivalis W83.Journal of bacteriology · 1999Article
- Porphyrin-mediated binding to hemoglobin by the HA2 domain of cysteine proteinases (gingipains) and hemagglutinins from the periodontal pathogen Porphyromonas gingivalis.Journal of bacteriology · 1999Article
- Life below the gum line: pathogenic mechanisms of Porphyromonas gingivalis.Microbiology and molecular biology reviews : MMBR · 1998Review
- The periodontopathogen Porphyromonas gingivalis binds iron protoporphyrin IX in the mu-oxo dimeric form: an oxidative buffer and possible pathogenic mechanism.The Biochemical journal · 1998Article
- Detection and comparison of specific hemin binding by Porphyromonas gingivalis and Prevotella intermedia.Journal of bacteriology · 1997Article
- Utilization of hemin and hemoglobin by Vibrio vulnificus biotype 2.Applied and environmental microbiology · 1996Article
- Heme binding by a bacterial repressor protein, the gene product of the ferric uptake regulation (fur) gene of Escherichia coli.Journal of protein chemistry · 1996Article
- A Legionella pneumophila gene that promotes hemin binding.Infection and immunity · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A 26-kDa outer membrane protein (Omp26) has been proposed to play a role in hemin acquisition by Porphyromonas gingivalis (T. E. Bramanti and S. C. Holt, J. Bacteriol. 174:5827-5839, 1992). We studied [55Fe]hemin uptake in P. gingivalis grown under conditions of hemin starvation (Omp26 expressed on the outer membrane surface) and hemin excess (Omp26 not expressed on surface). [55Fe]hemin uptake occurred rapidly in hemin-starved cells which incorporated up to 70% of total [55Fe]hemin within 3 min. P. gingivalis grown under hemin-starved conditions or treated with the iron chelator 2,2'-bipyridyl to induce an iron stress took up six times more [55Fe]hemin than hemin-excess-grown cells. Polyclonal monospecific anti-Omp26 antibody added to hemin-starved cells inhibited [55Fe]hemin uptake by more than 50%, whereas preimmune serum had no effect. [55Fe]hemin uptake in hemin-starved P. gingivalis was inhibited (36 to 67%) in the presence of equimolar amounts of unlabeled hemin, protoporphyrin IX, zinz protoporphyrin, and Congo red dye but was not inhibited in the presence of non-hemin-containing iron sources. Heat shock treatment (45 degrees C) of hemin-excess-grown P. gingivalis (which cases translocation of Omp26 to the surface) increased [55Fe]hemin uptake by threefold after 3 min in comparison with cells grown at 37 degrees C. However, no [55Fe] hemin uptake beyond 3 min was observed in either hemin-excess-grown or hemin-starved cells exposed to heat shock. In experiments using heterobifunctional cross-linker analysis, hemin and selected porphyrins were cross-linked to Omp26 in hemin-starved P. gingivalis, but no cross-linking was seen with hemin-excess-grown cells. However, cross-linking of hemin to Omp26 was observed after heat shock treatment of hemin-excess-grown cells. Finally, anti-Omp26 antibody inhibited cross-linked of hemin to Omp26. These findings indicate that hemin binding and transport into P.gingivalis cell mediated by Omp26.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.