ArticleThe EMBO journal1993
Nuclear Ca2+ concentration measured with specifically targeted recombinant aequorin.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 200 citations in OpenAlex.
- Amyloid β-Oligomers Inhibit the Nuclear CaAntioxidants (Basel, Switzerland) · 2023Article
- Generation and Characterization of a New FRET-Based CaInternational journal of molecular sciences · 2021Article
- Bioluminescent Sensors for CaFrontiers in bioengineering and biotechnology · 2021Article
- Calcium signaling: breast cancer's approach to manipulation of cellular circuitry.Biophysical reviews · 2020Review
- Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology.Nature communications · 2020Article
- Live Mitochondrial or Cytosolic Calcium Imaging Using Genetically-encoded Cameleon Indicator in Mammalian Cells.Bio-protocol · 2020Article
- TheInternational journal of molecular sciences · 2018Review
- Bioluminescence Assays for Monitoring Chondrogenic Differentiation and Cartilage Regeneration.Sensors (Basel, Switzerland) · 2017Review
- One-Dimensional Search Dynamics of Tumor Suppressor p53 Regulated by a Disordered C-Terminal Domain.Biophysical journal · 2017Article
- Exploring cells with targeted biosensors.The Journal of general physiology · 2017Review
- Nuclear pores enable sustained perinuclear calcium oscillations.BMC systems biology · 2016Article
- Opposing calcium-dependent signalling pathways control skeletal muscle differentiation by regulating a chromatin remodelling enzyme.Nature communications · 2015Article
- Nuclear calcium in cardiac myocytes.Journal of cardiovascular pharmacology · 2015Review
- Decoding calcium signaling across the nucleus.Physiology (Bethesda, Md.) · 2014Review
- GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes.Nature protocols · 2013Article
- Nuclear calcium signalling in the regulation of brain function.Nature reviews. Neuroscience · 2013Review
- Nucleoplasmic calcium signaling and cell proliferation: calcium signaling in the nucleus.Cell communication and signaling : CCS · 2013Article
- Calcium signaling in the liver.Comprehensive Physiology · 2013Review
- Nanodomain Ca²⁺ of Ca²⁺ channels detected by a tethered genetically encoded Ca²⁺ sensor.Nature communications · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Activation of nuclear transcription factors, breakdown of nuclear envelope and apoptosis represent a group of nuclear events thought to be modulated by changes in nucleoplasmic Ca2+ concentration, [Ca2+]n. Direct evidence for, or against, this possibility has been, however, difficult to obtain because measurements of [Ca2+]n are hampered by major technical problems. Here we describe a new approach for selectively monitoring Ca2+ concentrations inside the nucleus of living cells, which is based on the construction of a chimeric cDNA encoding a fusion protein composed of the photoprotein aequorin and a nuclear translocation signal derived from the rat glucocorticoid receptor. This modified aequorin (nuAEQ), stably expressed in HeLa cells, was largely confined to the nucleoplasm and thus utilized for monitoring [Ca2+]n in intact cells. No significant differences were observed between [Ca2+]n and cytosolic Ca2+ concentration ([Ca2+]i) under resting conditions. Upon stimulation of surface receptors linked to inositol-1,4,5-trisphosphate (InsP3) generation, and thus to intracellular Ca2+ signalling, the kinetics of [Ca2+]i and [Ca2+]n increases were indistinguishable. However, for the same rise in [Ca2+]i, the amplitude of [Ca2+]n increase was larger when evoked by Ca2+ mobilization from internal stores than when induced by Ca2+ influx across the plasma membrane. The functional significance of these transient nucleus-cytosol Ca2+ gradients is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.