ArticleThe EMBO journal1993
Mutations of the intronic IgH enhancer and its flanking sequences differentially affect accessibility of the JH locus.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 187 citations in OpenAlex.
- Article
- Recombination may occur in the absence of transcription in the immunoglobulin heavy chain recombination centre.Nucleic acids research · 2020Article
- Insertion of an imprinted insulator into the IgH locus reveals developmentally regulated, transcription-dependent control of V(D)J recombination.Molecular and cellular biology · 2015Article
- The dynamic architectural and epigenetic nuclear landscape: developing the genomic almanac of biology and disease.Journal of cellular physiology · 2014Review
- Germline deletion of Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Dynamic changes in binding of immunoglobulin heavy chain 3' regulatory region to protein factors during class switching.The Journal of biological chemistry · 2011Article
- The center of accessibility: Dβ control of V(D)J recombination.Archivum immunologiae et therapiae experimentalis · 2010Review
- Comparison of identical and functional Igh alleles reveals a nonessential role for Eμ in somatic hypermutation and class-switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Endothelial SUR-8 acts in an ERK-independent pathway during atrioventricular cushion development.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- CD4+CD25+ regulatory T cells in autoimmune arthritis.Immunological reviews · 2010Review
- A role for the IgH intronic enhancer E mu in enforcing allelic exclusion.The Journal of experimental medicine · 2009Article
- Requirement for enhancer specificity in immunoglobulin heavy chain locus regulation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Cis-regulatory elements and epigenetic changes control genomic rearrangements of the IgH locus.Advances in immunology · 2008Review
- Chromosomal position of a VH gene segment determines its activation and inactivation as a substrate for V(D)J recombination.The Journal of experimental medicine · 2007Article
- In a model of immunoglobulin heavy-chain (IGH)/MYC translocation, the Igh 3' regulatory region induces MYC expression at the immature stage of B cell development.Genes, chromosomes & cancer · 2007Article
- Transcription of a productively rearranged Ig VDJC alpha does not require the presence of HS4 in the IgH 3' regulatory region.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci.The Journal of experimental medicine · 2006Article
- Elucidation of IgH intronic enhancer functions via germ-line deletion.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesis.The Journal of cell biology · 2005Article
- The T-cell receptor beta variable gene promoter is required for efficient V beta rearrangement but not allelic exclusion.Molecular and cellular biology · 2004Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
To investigate the role of intronic immunoglobulin heavy chain (IgH) enhancer (E mu) in generating accessibility of the JH locus for VDJ recombination, we generated ES cells in which E mu or its flanking sequences were mutated by replacement with or insertion of an expressed neor gene. Heterozygous mutant ES cells were used to generate chimeric mice from which pre-B cell lines were derived by transformation of bone marrow cells with Abelson murine leukemia virus (A-MuLV). Comparison of the rearrangement status of the normal and mutated alleles in individual pre-B cell lines allowed us to assay for cis-acting effects of the mutations. Replacement of a 700 bp region immediately downstream from the core E mu [which includes part of the 3' matrix associated region (MAR) and the I mu exon] had no obvious effect on rearrangement of the targeted allele, indicating that insertion of a transcribed neor gene into the JH-C mu intron does not affect JH accessibility. In contrast, replacement of an overlapping 1 kb DNA fragment that contains the E mu resulted in a dramatic cis-acting inhibition of rearrangement, demethylation and germline transcription of the associated JH locus. Surprisingly, insertion of the neor gene into the 5' MAR sequence approximately 100 bp upstream of the core E mu also dramatically decreased recombination of the linked JH locus; but, in many lines, did not prevent demethylation of this locus. We conclude that integrity of the E mu and upstream flanking sequences is required for efficient rearrangement of the JH locus and that demethylation of this locus, per se, does not necessarily make it a good substrate for VDJ recombination.
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