ArticleThe EMBO journal1993
DNA and redox state induced conformational changes in the DNA-binding domain of the Myb oncoprotein.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 145 citations in OpenAlex.
- MYB: A Key Transcription Factor in the Hematopoietic System Subject to Many Levels of Control.Advances in experimental medicine and biology · 2024Review
- DNA-binding function of c-Myb R2R3 around thermal denaturation temperature.Biophysics and physicobiology · 2021Article
- Independent Evolution of the MYB Family in Brown Algae.Frontiers in genetics · 2021Article
- A c-Myb mutant causes deregulated differentiation due to impaired histone binding and abrogated pioneer factor function.Nucleic acids research · 2017Article
- PETAL LOSS and ROXY1 Interact to Limit Growth Within and between Sepals But to Promote Petal Initiation inFrontiers in plant science · 2017Article
- c-Myb Binding Sites in Haematopoietic Chromatin Landscapes.PloS one · 2015Article
- Dominant repression by Arabidopsis transcription factor MYB44 causes oxidative damage and hypersensitivity to abiotic stress.International journal of molecular sciences · 2014Article
- Redox regulation of T-cell function: from molecular mechanisms to significance in human health and disease.Antioxidants & redox signaling · 2013Review
- FlbD, a Myb transcription factor of Aspergillus nidulans, is uniquely involved in both asexual and sexual differentiation.Eukaryotic cell · 2012Article
- Inhibition of Myb-dependent gene expression by the sesquiterpene lactone mexicanin-I.Leukemia · 2012Article
- Alternative SAIL-Trp for robust aromatic signal assignment and determination of the χ(2) conformation by intra-residue NOEs.Journal of biomolecular NMR · 2011Article
- Animal-specific C-terminal domain links myeloblastosis oncoprotein (Myb) to an ancient repressor complex.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress.BMC plant biology · 2010Article
- An early response regulatory cluster induced by low temperature and hydrogen peroxide in seedlings of chilling-tolerant japonica rice.BMC genomics · 2007Article
- The effect of Euryale ferox (Makhana), an herb of aquatic origin, on myocardial ischemic reperfusion injury.Molecular and cellular biochemistry · 2006Article
- Selective inhibition of c-Myb DNA-binding by RNA polymers.BMC biochemistry · 2004Article
- Thioredoxin as a neurotrophic cofactor and an important regulator of neuroprotection.Molecular neurobiology · 2004Review
- Redox regulation of DNA binding activity of DREF (DNA replication-related element binding factor) in Drosophila.The Biochemical journal · 2004Article
- Internal thiols and reactive oxygen species in candidacidal activity exerted by an N-terminal peptide of human lactoferrin.Antimicrobial agents and chemotherapy · 2002Article
- Regulation of the Arabidopsis transcriptome by oxidative stress.Plant physiology · 2001Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DNA-binding domain of the oncoprotein Myb comprises three imperfect repeats, R1, R2 and R3. Only R2 and R3 are required for sequence-specific DNA-binding. Both are assumed to contain helix-turn-helix (HTH)-related motifs, but multidimensional heteronuclear NMR spectroscopy revealed a disordered structure in R2 where the second HTH helix was predicted [Jamin et al. (1993) Eur. J. Biochem., 216, 147-154]. We propose that the disordered region folds into a 'recognition' helix and generates a full HTH-related motif upon binding to DNA. This would move Cys43 into the hydrophobic core of R2. We observed that Cys43 was accessible to N-ethylmaleimide alkylation in the free protein, but inaccessible in the DNA complex. Mutant proteins with charged (C43D) or polar (C43S) side chains in position 43 bound DNA with reduced affinity, while hydrophobic replacements (C43A, C43V and C43I) gave unaltered or improved DNA-binding. Specific DNA-binding enhanced protease resistance dramatically. Fluorescence emission spectra and quenching experiments supported a DNA-induced conformational change. Moreover, reversible oxidation of Cys43 had an effect similar to the inactivating C43D mutation. The highly oxidizable Cys43 could function as a molecular sensor for a redox regulatory mechanism turning specific DNA-binding on or off by controlling the DNA-induced conformational change in R2.
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