ArticleThe EMBO journal1993
Promoter, enhancer and silencer elements regulate rearrangement of an immunoglobulin transgene.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 82 citations in OpenAlex.
- RecQL5 promotes genome stabilization through two parallel mechanisms--interacting with RNA polymerase II and acting as a helicase.Molecular and cellular biology · 2010Article
- Functional analysis of histone methyltransferase g9a in B and T lymphocytes.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.The Journal of experimental medicine · 2006Article
- Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line.Nucleic acids research · 2006Article
- Activation of a DNA damage checkpoint response in a TAF1-defective cell line.Molecular and cellular biology · 2004Article
- Transcription and double-strand breaks induce similar mitotic recombination events in Saccharomyces cerevisiae.Genetics · 2002Article
- The connection between transcription and genomic instability.The EMBO journal · 2002Review
- Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Article
- A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae.The EMBO journal · 2000Article
- Definition of a T-cell receptor beta gene core enhancer of V(D)J recombination by transgenic mapping.Molecular and cellular biology · 2000Article
- A targeted deletion of a region upstream from the Jkappa cluster impairs kappa chain rearrangement in cis in mice and in the 103/bcl2 cell line.The Journal of experimental medicine · 1999Article
- A novel yeast gene, THO2, is involved in RNA pol II transcription and provides new evidence for transcriptional elongation-associated recombination.The EMBO journal · 1998Article
- The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability.Genes & development · 1997Article
- Enhancer control of local accessibility to V(D)J recombinase.Molecular and cellular biology · 1997Article
- Coordinate transcription and V(D)J recombination of the kappa immunoglobulin light-chain locus: NF-kappaB-dependent and -independent pathways of activation.Molecular and cellular biology · 1997Article
- V(D)J recombination frequency is affected by the sequence interposed between a pair of recombination signals: sequence comparison reveals a putative recombinational enhancer element.Nucleic acids research · 1997Article
- Recombination between DNA repeats in yeast hpr1delta cells is linked to transcription elongation.The EMBO journal · 1997Article
- Regulation of T cell receptor delta gene rearrangement by CBF/PEBP2.The Journal of experimental medicine · 1997Article
- Domains of the TCR beta-chain required for early thymocyte development.The Journal of experimental medicine · 1996Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chicken Ig lambda light chain locus is composed of a single V gene closely linked (1.8 kb) to a single J-C unit in its natural configuration. In mice transgenic for this locus, the transgene becomes rearranged in B cells and to a much lesser extent in T cells. Modifications were introduced in the transgene in order to characterize elements which target the recombinase to the Ig loci. In the absence of either the promoter or the enhancer located 3' of C lambda, rearrangement of the transgene is reduced 20- to 100-fold. Moreover, rearrangement is increased 5-fold when the DNA segment between V lambda and J lambda ('Uo segment'), which is deleted during the joining process, is replaced by a neutral DNA segment of equal length. The Uo segment behaved as a strong transcriptional silencer when tested in a CAT assay in vitro. Control transgenic mice harbouring only the two 3 bp mutations that introduced restriction sites at both ends of the Uo segment to allow for its replacement were also analysed. Rearrangement was reduced 10- to 100-fold in B cells from such transgenic lines. A model is proposed whereby the sites of these two mutations would function by counteracting transiently the repressing effect of the silencer, thus giving access of the chicken light chain locus to the recombinase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.