ArticleThe EMBO journal1993
The role of the 70 kDa subunit of human DNA polymerase alpha in DNA replication.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 124 citations in OpenAlex.
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- Telomere C-Strand Fill-In Machinery: New Insights into the Human CST-DNA Polymerase Alpha-Primase Structures and Functions.Sub-cellular biochemistry · 2024Review
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- Genetic Reprogramming of Positional Memory in a Regenerating Appendage.Current biology : CB · 2019Article
- Plant DNA Polymerases.International journal of molecular sciences · 2019Review
- Knockdown of POLA2 increases gemcitabine resistance in lung cancer cells.BMC genomics · 2016Article
- Role for RNA:DNA hybrids in origin-independent replication priming in a eukaryotic system.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Structural basis for the interaction of a hexameric replicative helicase with the regulatory subunit of human DNA polymerase α-primase.The Journal of biological chemistry · 2012Article
- A specific docking site for DNA polymerase {alpha}-primase on the SV40 helicase is required for viral primosome activity, but helicase activity is dispensable.The Journal of biological chemistry · 2010Article
- Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome.The Journal of biological chemistry · 2010Article
- Characterization of RNA primers synthesized by the human breast cancer cell DNA synthesome.Journal of cellular biochemistry · 2009Article
- Domain architecture and biochemical characterization of vertebrate Mcm10.The Journal of biological chemistry · 2008Article
- The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.Molecular and cellular biology · 2004Article
- Pol12, the B subunit of DNA polymerase alpha, functions in both telomere capping and length regulation.Genes & development · 2004Article
- Assembly of the replication initiation complex on SV40 origin DNA.Nucleic acids research · 2004Article
- Subunit interactions in the assembly of Saccharomyces cerevisiae DNA polymerase alpha.Nucleic acids research · 2003Article
- Role of the p68 subunit of human DNA polymerase alpha-primase in simian virus 40 DNA replication.Molecular and cellular biology · 2002Article
- Mutational analysis of simian virus 40 T-antigen primosome activities in viral DNA replication.Journal of virology · 2002Article
- Amino acids 257 to 288 of mouse p48 control the cooperation of polyomavirus large T antigen, replication protein A, and DNA polymerase alpha-primase to synthesize DNA in vitro.Journal of virology · 2001Article
- Two immunologically distinct human DNA polymerase alpha-primase subpopulations are involved in cellular DNA replication.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
DNA polymerase alpha is the only enzyme in eukaryotic cells capable of starting DNA chains de novo and is required for the initiation of SV40 DNA replication in vitro. We have cloned the 70 kDa subunit of human DNA polymerase alpha (hereafter referred to as the B subunit) and expressed it as a fusion protein in bacteria. The purified fusion protein forms a stable complex with SV40 T antigen, both in solution and when T antigen is bound to the SV40 origin of DNA replication. Analysis of mutant forms of the B subunit indicates that the N-terminal 240 amino acids are sufficient to mediate complex formation. The B subunit fusion protein promotes formation of a complex containing T antigen and the catalytic subunit (subunit A) of DNA polymerase alpha, suggesting that it serves to tether the two proteins. These physical interactions are functionally significant, since the ability of T antigen to stimulate the activity of the catalytic subunit of DNA polymerase alpha is highly dependent upon the B subunit. We suggest that the interactions mediated by the B subunit play an important role in SV40 DNA replication by promoting DNA chain initiation at the origin and/or facilitating the subsequent priming and synthesis of DNA chains on the lagging strand template. The protein may play similar roles in cellular DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.