ArticleThe EMBO journal1993
Disruption of the nucleosomes at the replication fork.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 127 citations in OpenAlex.
- Psoralen Crosslinking-Chromatin Endogenous Cleavage Assay to Examine Histone DNA Interactions of Active and Inactive rRNA Genes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Roles of Histone H2B, H3 and H4 Variants in Cancer Development and Prognosis.International journal of molecular sciences · 2024Review
- Role of Chromatin Replication in Transcriptional Plasticity, Cell Differentiation and Disease.Genes · 2022Review
- Parental nucleosome segregation and the inheritance of cellular identity.Nature reviews. Genetics · 2021Review
- Replication-Coupled Chromatin Remodeling: An Overview of Disassembly and Assembly of Chromatin during Replication.International journal of molecular sciences · 2021Review
- The replisome guides nucleosome assembly during DNA replication.Cell & bioscience · 2020Review
- The nucleosome core particle remembers its position through DNA replication and RNA transcription.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nucleosomes remember where they were.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication.Molecular cell · 2018Article
- Major Determinants of Nucleosome Positioning.Biophysical journal · 2018Review
- Article
- Capitalizing on disaster: Establishing chromatin specificity behind the replication fork.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- DNA looping mediates nucleosome transfer.Nature communications · 2016Article
- Chromatin assembly: Journey to the CENter of the chromosome.The Journal of cell biology · 2016Review
- Maintaining Epigenetic Inheritance During DNA Replication in Plants.Frontiers in plant science · 2016Review
- Review
- High-resolution chromatin dynamics during a yeast stress response.Molecular cell · 2015Article
- Replication-guided nucleosome packing and nucleosome breathing expedite the formation of dense arrays.Nucleic acids research · 2014Article
- Chromatin dynamics at the replication fork: there's more to life than histones.Current opinion in genetics & development · 2013Review
- Structural plasticity of histones H3-H4 facilitates their allosteric exchange between RbAp48 and ASF1.Nature structural & molecular biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fate of parental nucleosomes during chromatin replication was studied in vitro using in vitro assembled chromatin containing the whole SV40 genome as well as salt-treated and native SV40 minichromosomes. In vitro assembled minichromosomes were able to replicate efficiently in vitro, when the DNA was preincubated with T-antigen, a cytosolic S100 extract and three deoxynucleoside triphosphates prior to chromatin assembly, indicating that the origin has to be free of nucleosomes for replication initiation. The chromatin structure of the newly synthesized daughter strands in replicating molecules was analysed by psoralen cross-linking of the DNA and by micrococcal nuclease digestion. A 5- and 10-fold excess of protein-free competitor DNA present during minichromosome replication traps the segregating histones. In opposition to published data this suggests that the parental histones remain only loosely or not attached to the DNA in the region of the replication fork. Replication in the putative absence of free histones shows that a subnucleosomal particle is randomly assembled on the daughter strands. The data are compatible with the formation of a H3/H4 tetramer complex under these conditions, supporting the notion that under physiological conditions nucleosome core assembly on the newly synthesized daughter strands occurs by the binding of H2A/H2B dimers to a H3/H4 tetramer complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.