ArticleThe EMBO journal1993
dUTP pyrophosphatase is an essential enzyme in Saccharomyces cerevisiae.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 221 citations in OpenAlex.
- Functional characterization ofThe veterinary quarterly · 2026Article
- dUTPase modulates mycobacterial homologous recombination and interacts with the AdnAB helicase-nuclease.Nucleic acids research · 2026Article
- Is dUTPase Enzymatic Activity Truly Essential for Viability?International journal of molecular sciences · 2025Review
- Auxotrophy-based curation improves the consensus genome-scale metabolic model of yeast.Synthetic and systems biotechnology · 2024Article
- The homodimerization domain of the Stl repressor is crucial for efficient inhibition of mycobacterial dUTPase.Scientific reports · 2024Article
- Bacteriophage T5 dUTPase: Combination of Common Enzymatic and Novel Functions.International journal of molecular sciences · 2024Article
- Cross-feeding promotes heterogeneity within yeast cell populations.Nature communications · 2024Article
- Discovery of two new isoforms of the human DUT gene.Scientific reports · 2023Article
- Viral dUTPases: Modulators of Innate Immunity.Biomolecules · 2022Review
- Identification of genetic elements required forFrontiers in microbiology · 2022Article
- Redox status of cysteines does not alter functional properties of human dUTPase but the Y54C mutation involved in monogenic diabetes decreases protein stability.Scientific reports · 2021Article
- dUTPase inhibition confers susceptibility to a thymidylate synthase inhibitor in DNA-repair-defective human cancer cells.Cancer science · 2021Article
- The dUTPase of caprine arthritis-encephalitis virus negatively regulates interferon signaling pathway.Iranian journal of veterinary research · 2021Article
- Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio.Nucleic acids research · 2020Article
- Exploiting the Autozygome to Support Previously Published Mendelian Gene-Disease Associations: An Update.Frontiers in genetics · 2020Article
- Validation of Plasmodium falciparum dUTPase as the target of 5'-tritylated deoxyuridine analogues with anti-malarial activity.Malaria journal · 2019Article
- The Role of a Key Amino Acid Position in Species-Specific Proteinaceous dUTPase Inhibition.Biomolecules · 2019Article
- CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early Embryonic Lethality.Biomolecules · 2019Article
- The etiology of uracil residues in the Saccharomyces cerevisiae genomic DNA.Current genetics · 2019Review
- Genetic variants of the dUTPase-encoding gene DUT increase HR-HPV infection rate and cervical squamous cell carcinoma risk.Scientific reports · 2019Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
dUTP pyrophosphatase (dUTPase; EC 3.6.1.23) catalyses the hydrolysis of dUTP to dUMP and PPi and thereby prevents the incorporation of uracil into DNA during replication. Although it is widely believed that dUTPase is essential for cell viability because of this role, direct evidence supporting this assumption has not been presented for any eukaryotic system. We have analysed the role of dUTPase (DUT1) in the life cycle of yeast. Using gene disruption and tetrad analysis, we find that DUT1 is necessary for the viability of S. cerevisiae; however, under certain conditions dut1 null mutants survive if supplied with exogenous thymidylate (dTMP). Analyses with isogenic uracil-DNA-glycosylase (UNG1) deficient or proficient strains indicate that in the absence of dUTPase, cell death results from the incorporation of uracil into DNA and the attempted repair of this damage by UNG1-mediated excision repair. However, in dut1 ung1 double mutants, starvation for dTMP causes dividing cells to arrest and die in all phases of the cell cycle. This latter effect suggests that the extensive stable substitution of uracil for thymine in DNA leads to a general failure in macromolecular synthesis. These results are in general agreement with previous models in thymine-less death that implicate dUTP metabolism. They also suggest an alternative approach for chemotherapeutic drug design.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.