ArticleThe EMBO journal1993
The alpha-helical rod domain of human lamins A and C contains a chromatin binding site.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 157 citations in OpenAlex.
- The molecular basis of lamin-specific chromatin interactions.Nature structural & molecular biology · 2025Article
- Review
- Epigenetics inCells · 2023Review
- Slower diffusion and anomalous association of R453W lamin A protein alter nuclear architecture in AD-EDMD.RSC advances · 2022Article
- Muscle Enriched Lamin Interacting Protein (Cells · 2021Article
- Review
- Chemical Decorations of "MARs" Residents in Orchestrating Eukaryotic Gene Regulation.Frontiers in cell and developmental biology · 2020Review
- Lamin A/C and Emerin depletion impacts chromatin organization and dynamics in the interphase nucleus.BMC molecular and cell biology · 2019Article
- Spatial chromatin organization and gene regulation at the nuclear lamina.Current opinion in genetics & development · 2019Review
- Article
- Non-farnesylated B-type lamin can tether chromatin inside the nucleus and its chromatin interaction requires the Ig-fold region.Chromosoma · 2017Article
- Physiological and Pathological Aging Affects Chromatin Dynamics, Structure and Function at the Nuclear Edge.Frontiers in genetics · 2016Review
- Review
- The different function of single phosphorylation sites of Drosophila melanogaster lamin Dm and lamin C.PloS one · 2012Article
- Article
- Uncoordinated transcription and compromised muscle function in the lmna-null mouse model of Emery- Emery-Dreyfuss muscular dystrophy.PloS one · 2011Article
- Novel roles for A-type lamins in telomere biology and the DNA damage response pathway.The EMBO journal · 2009Article
- Nuclear lamins and chromatin: when structure meets function.Advances in enzyme regulation · 2009Review
- Nuclear localization and cytosolic overexpression of LASP-1 correlates with tumor size and nodal-positivity of human breast carcinoma.BMC cancer · 2007Article
- Proteasome-mediated degradation of integral inner nuclear membrane protein emerin in fibroblasts lacking A-type lamins.Biochemical and biophysical research communications · 2006Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined regions of human lamins A and C involved in binding to surfaces of mitotic chromosomes. An Escherichia coli expression system was used to produce full-length lamin A and lamin C, and truncated lamins retaining the central alpha-helical rod domain (residues 34-388) but lacking various amounts of the amino-terminal 'head' and carboxy-terminal 'tail' domains. We found that lamin A, lamin C and lamin fragments lacking the head domain and tail sequences distal to residue 431 efficiently assembled into paracrystals and strongly associated with mitotic chromosomes. Furthermore, the lamin rod domain also associated with chromosomes, although efficient chromosome coating required the pH 5-6 conditions needed to assemble the rod into higher order structures. Biochemical assays showed that chromosomes substantially reduced the critical concentration for assembly of lamin polypeptides into pelletable structures. Association of the lamin rod with chromosomes was abolished by pretrypsinization of chromosomes, and was not seen for vimentin (which possesses a similar rod domain). These data demonstrate that the alpha-helical rod of lamins A and C contains a specific chromosome binding site. Hence, the central rod domain of intermediate filament proteins can be involved in interactions with other cellular structures as well as in filament assembly.
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