ArticlePlant molecular biology1993
Plant viral leaders influence expression of a reporter gene in tobacco.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Identification of an internal RNA element essential for replication and translational enhancement of tobacco necrosis virus A(C).PloS one · 2013Article
- Design of gene constructs for transgenic maize.Methods in molecular biology (Clifton, N.J.) · 2009Review
- In vivo characterization of plant promoter element interaction using synthetic promoters.Transgenic research · 2008Article
- Transgenic plants for animal health: plant-made vaccine antigens for animal infectious disease control.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2008Article
- Improvement of the pBI121 plant expression vector by leader replacement with a sequence combining a poly(CAA) and a CT motif.Transgenic research · 2007Article
- Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude.BMC biotechnology · 2004Article
- Cestrum yellow leaf curling virus (CmYLCV) promoter: a new strong constitutive promoter for heterologous gene expression in a wide variety of crops.Plant molecular biology · 2003Article
- Promoters for pregenomic RNA of banana streak badnavirus are active for transgene expression in monocot and dicot plants.Plant molecular biology · 2001Article
- Enhanced synthesis of choline and glycine betaine in transgenic tobacco plants that overexpress phosphoethanolamine N-methyltransferase.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Heat shock protein HSP101 binds to the Fed-1 internal light regulator y element and mediates its high translational activity.The Plant cell · 2000Article
- Structure and promoter/leader deletion analysis of mirabilis mosaic virus (MMV) full-length transcript promoter in transgenic plants.Plant molecular biology · 1999Article
- Forced evolution reveals the importance of short open reading frame A and secondary structure in the cauliflower mosaic virus 35S RNA leader.Journal of virology · 1998Article
- Promoter/leader deletion analysis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains.Transgenic research · 1997Article
- Efficient transcription from the rice tungro bacilliform virus promoter requires elements downstream of the transcription start site.Journal of virology · 1996Article
- RNA structure and the regulation of gene expression.Plant molecular biology · 1996Review
- Translation in plants--rules and exceptions.Plant molecular biology · 1996Review
- Translational control of cellular and viral mRNAs.Plant molecular biology · 1996Review
- Isolation and expression in transgenic tobacco and rice plants, of the cassava vein mosaic virus (CVMV) promoter.Plant molecular biology · 1996Article
- Comparison of different constitutive and inducible promoters for the overexpression of transgenes in Arabidopsis thaliana.Plant molecular biology · 1995Article
- The sequence surrounding the translation initiation codon of the pea plastocyanin gene increases translational efficiency of a reporter gene.Plant molecular biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower mosaic virus (CaMV) 35S RNA leader, were compared. Viral leader constructs employing the 35S promoter and the reporter beta-glucuronidase (GUS) were tested by electroporation into tobacco mesophyll protoplasts and against a cointroduced chloramphenicol acetyl transferase (CAT) gene in transgenic tobacco leaves. In the transient assay system, GUS activities from the viral leaders were compared with those from either a short, random leader or a translational fusion of the CaMV 19S RNA ORF VI to GUS. A two- to-three-fold enhanced level of expression resulted when these leaders were substituted with either the 35S RNA or the TMV leader sequences. This enhancement was further increased, to four- to five-fold, by inclusion of four or seven of the bases from the 35S transcription initiation site adjacent to the TMV leader. In transgenic tobacco the improved GUS levels were maintained from constructs including either the TMV leader (eight-fold) or this sequence with the addition of the 35S transcription initiation site bases (ten-fold). A comparison of GUS enzyme amounts with GUS mRNA amounts, using the CAT gene as an internal standard, revealed that TMV leader-bearing mRNA was translated from four- to six-fold more efficiently than the random leader control.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.