ArticleApplied and environmental microbiology1993
Construction of a recombinant wine yeast strain expressing beta-(1,4)-endoglucanase and its use in microvinification processes.
Article in Applied and environmental microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Truth in wine yeast.Microbial biotechnology · 2022Review
- Screening of enzymatic activities within different enological non-Journal of food science and technology · 2017Article
- Cultivable psychrotolerant yeasts associated with Antarctic marine sponges.World journal of microbiology & biotechnology · 2013Article
- Flavour-active wine yeasts.Applied microbiology and biotechnology · 2012Review
- Wine flavor and aroma.Journal of industrial microbiology & biotechnology · 2011Review
- Construction of sterile ime1Delta-transgenic Saccharomyces cerevisiae wine yeasts unable to disseminate in nature.Applied and environmental microbiology · 2008Article
- Comparison of two alternative dominant selectable markers for wine yeast transformation.Applied and environmental microbiology · 2004Article
- Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an alpha-L-rhamnosidase of enological interest.Applied and environmental microbiology · 2003Article
- Microbial cellulose utilization: fundamentals and biotechnology.Microbiology and molecular biology reviews : MMBR · 2002Review
- Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes.Applied and environmental microbiology · 1996Article
- Development of a transformation system for Trichoderma longibrachiatum and its use for constructing multicopy transformants for the egl1 gene.Applied microbiology and biotechnology · 1994Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A genetic transformation system for an industrial wine yeast strain is presented here. The system is based on the acquisition of cycloheximide resistance and is a direct adaptation of a previously published procedure for brewing yeasts (L. Del Pozo, D. Abarca, M. G. Claros, and A. Jiménez, Curr. Genet. 19:353-358, 1991). Transformants arose at an optimal frequency of 0.5 transformant per microgram of DNA, are stable in the absence of selective pressure, and produce wine in the same way as the untransformed industrial strain. By using this transformation protocol, a filamentous fungal beta-(1,4)-endoglucanase gene has been expressed in an industrial wine yeast under the control of the yeast actin gene promoter. Endoglucanolytic wine yeast secretes the fungal enzyme to the must, producing a wine with an increased fruity aroma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.