ArticleApplied and environmental microbiology1993
A simple RNA probe system for analysis of Listeria monocytogenes polymerase chain reaction products.
Article in Applied and environmental microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Genetic diversity ofAIMS microbiology · 2024Article
- Prevalence of Very Low Numbers of Potential Pathogenic Isolates of Yersiniaenterocolitica and Yersinia intermedia in Traditional Fast Foods of India.Indian journal of microbiology · 2011Article
- Effectiveness of steam pasteurization in controlling microbiological hazards of cull cow carcasses in a commercial plant.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2005Article
- Methodologies for the characterization of microbes in industrial environments: a review.Journal of industrial microbiology & biotechnology · 2003Review
- A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences.Applied and environmental microbiology · 1997Article
- Transcriptional enhancement of the Listeria monocytogenes PCR and simple immunoenzymatic assay of the product using anti-RNA:DNA antibodies.Applied and environmental microbiology · 1994Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synthesis of an RNA probe specific for the hlyA gene of Listeria monocytogenes by in vitro transcription from a polymerase chain reaction (PCR)-generated template incorporating bacteriophage T7 promoter sequences is described. This simple method produced a high yield of RNA which hybridized specifically with hlyA PCR products on a membrane, resulting in RNA-DNA hybrids which were detected by an immunoenzymatic assay with an anti-RNA-DNA hybrid antibody. The RNA probe hybridization system was more sensitive in the analysis of the PCR products than was the conventional agarose gel electrophoresis method. When applied to the analysis of PCR samples from cultures of various Listeria and non-Listeria organisms, the RNA probe was reactive in the assay of 62 different L. monocytogenes isolates but not other Listeria species. Among the non-Listeria organisms tested, only Enterococcus faecalis gave a weak positive reaction with more than 10(9) cells per ml. This reactivity disappeared at lower cell densities. This strategy for the synthesis and application of RNA probes should facilitate the analysis of PCR products in the detection of L. monocytogenes and possibly other food pathogens.
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