ArticleThe American journal of pathology1993
Differential expression of tumor necrosis factor and interleukin-6 by peritoneal macrophages in vivo and in culture.
Article in The American journal of pathology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A brain cytokine-independent switch in cortical activity marks the onset of sickness behavior triggered by acute peripheral inflammation.Journal of neuroinflammation · 2023Article
- Challenge model of TNFJournal of pharmacokinetics and pharmacodynamics · 2019Article
- Identification and characterization of a novel anti-inflammatory lipid isolated from Mycobacterium vaccae, a soil-derived bacterium with immunoregulatory and stress resilience properties.Psychopharmacology · 2019Article
- The Autoantigenic Proinsulin B-Chain Peptide B11-23 Synergises with the 70 kDa Heat Shock Protein DnaK in Macrophage Stimulation.Journal of diabetes research · 2018Article
- Article
- Mast cells aggravate sepsis by inhibiting peritoneal macrophage phagocytosis.The Journal of clinical investigation · 2014Article
- Reducing LPS content in cockroach allergens increases pulmonary cytokine production without increasing inflammation: a randomized laboratory study.BMC pulmonary medicine · 2011Article
- Potentiation of Innate Immunity by β-Glucans.Mycobiology · 2010Article
- Acute pulmonary lipopolysaccharide tolerance decreases TNF-alpha without reducing neutrophil recruitment.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Tumor necrosis factor inhibitors for the treatment of asthma.Current allergy and asthma reports · 2007Review
- The net immunologic advantage of laparoscopic surgery.Surgical endoscopy · 2004Review
- Metallothionein modulates lipopolysaccharide-stimulated tumour necrosis factor expression in mouse peritoneal macrophages.The Biochemical journal · 2002Article
- Ratio of local to systemic chemokine concentrations regulates neutrophil recruitment.The American journal of pathology · 2001Article
- Modulation of endotoxin- and enterotoxin-induced cytokine release by in vivo treatment with beta-(1,6)-branched beta-(1,3)-glucan.Infection and immunity · 1999Article
- Lipopolysaccharide-induced cytokine cascade and lethality in LT alpha/TNF alpha-deficient mice.Molecular medicine (Cambridge, Mass.) · 1997Article
- Article
- Immunological priming attenuates the in vivo pathophysiological response to lipopolysaccharide. Comparison of cytokine production, tissue injury, and lethality in complete Freund's adjuvant-primed mice and in unprimed mice.The American journal of pathology · 1994Article
- Functional and morphological effects of tumour necrosis factor alpha in an interleukin 6-producing pulmonary large cell carcinoma with sarcomatoid features.Virchows Archiv : an international journal of pathology · 1994Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To investigate the differences in cytokine regulation in vitro as compared to in vivo, we examined the synthesis of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) by peritoneal macrophages in response to lipopolysaccharide (LPS). Mice (CBA/J) were primed with an intraperitoneal injection of complete Freund's adjuvant and after 2 weeks, peritoneal cells were harvested for culture or mice were injected intraperitoneally with LPS for in vivo studies. In ascites fluid, TNF-alpha peaked 1 hour after LPS and returned to baseline levels by 4 hours. In contrast, TNF-alpha in the media reached maximum at 7 hours. Expression of TNF-alpha messenger (m)RNA in vivo was rapid but transient, as levels peaked at 15 minutes and returned to baseline 1 hour after LPS. In contrast, TNF-alpha mRNA in vitro became maximal at 1 hour, but remained elevated to 5 hours after LPS. In vivo, IL-6 in ascites fluid peaked at 2 hours, whereas in vitro, IL-6 continued increasing to 24 hours. In vivo, IL-6 mRNA reached maximum at 30 minutes, but fell below baseline by 1.5 hours after LPS. In contrast, IL-6 mRNA in vitro was sustained at maximal expression between 5 to 9 hours after LPS. These results demonstrate that both TNF-alpha and IL-6 synthesis is more rapid in vivo than in vitro. The rapid kinetics of cytokine expression in vivo must considered when designing strategies to inhibit cytokine action in vivo.
Indexed as
Identifiers
8214006PMC1887059What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.