ArticleJournal of virology1994
Subclonal components of consensus fitness in an RNA virus clone.
Article in Journal of virology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 138 citations in OpenAlex.
- Quasispecies theory and emerging viruses: challenges and applications.Npj viruses · 2024Review
- Evolutionary Adaptation of an RNA Bacteriophage to Repeated Freezing and Thawing Cycles.International journal of molecular sciences · 2024Article
- Unraveling the dynamics of hepatitis C virus adaptive mutations and their impact on antiviral responses in primary human hepatocytes.Journal of virology · 2024Article
- Quasispecies productivity.Die Naturwissenschaften · 2024Article
- Review
- A Two-Level, Intramutant Spectrum Haplotype Profile of Hepatitis C Virus Revealed by Self-Organized Maps.Microbiology spectrum · 2021Article
- Tradeoffs for a viral mutant with enhanced replication speed.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Intra-Population Competition during Adaptation to Increased Temperature in an RNA Bacteriophage.International journal of molecular sciences · 2021Article
- Role of mutational reversions and fitness restoration in Zika virus spread to the Americas.Nature communications · 2021Article
- They are what you eat: Shaping of viral populations through nutrition and consequences for virulence.PLoS pathogens · 2020Review
- Viral quasispecies.PLoS genetics · 2019Article
- Quasispecies and virus.European biophysics journal : EBJ · 2018Review
- Bacteria Facilitate Enteric Virus Co-infection of Mammalian Cells and Promote Genetic Recombination.Cell host & microbe · 2018Article
- Differences in adaptive dynamics determine the success of virus variants that propagate together.Virus evolution · 2018Article
- Adaptive multiscapes: an up-to-date metaphor to visualize molecular adaptation.Biology direct · 2017Article
- Genetic variation in fitness within a clonal population of a plant RNA virus.Virus evolution · 2016Article
- Viral quasispecies.Virology · 2015Review
- Nature and Extent of Genetic Diversity of Dengue Viruses Determined by 454 Pyrosequencing.PloS one · 2015Article
- Article
- Consequences of in vitro host shift for St. Louis encephalitis virus.The Journal of general virology · 2014Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Most RNA virus populations exhibit extremely high mutation frequencies which generate complex, genetically heterogeneous populations referred to as quasi-species. Previous work has shown that when a large spectrum of the quasi-species is transferred, natural selection operates, leading to elimination of noncompetitive (inferior) genomes and rapid gains in fitness. However, whenever the population is repeatedly reduced to a single virion, variable declines in fitness occur as predicted by the Muller's ratchet hypothesis. Here, we quantitated the fitness of 98 subclones isolated from an RNA virus clonal population. We found a normal distribution around a lower fitness, with the average subclone being less fit than the parental clonal population. This finding demonstrates the phenotypic diversity in RNA virus populations and shows that, as expected, a large fraction of mutations generated during virus replication is deleterious. This clarifies the operation of Muller's ratchet and illustrates why a large number of virions must be transferred for rapid fitness gains to occur. We also found that repeated genetic bottleneck passages can cause irregular stochastic declines in fitness, emphasizing again the phenotypic heterogeneity present in RNA virus populations. Finally, we found that following only 60 h of selection (15 passages in which virus yields were harvested after 4 h), RNA virus populations can undergo a 250% average increase in fitness, even on a host cell type to which they were already well adapted. This is a remarkable ability; in population biology, even a much lower fitness gain (e.g., 1 to 2%) can represent a highly significant reproductive advantage. We discuss the biological implications of these findings for the natural transmission and pathogenesis of RNA viruses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.