ArticleJournal of virology1994
Identification of sequence elements in the human cytomegalovirus DNA polymerase gene promoter required for activation by viral gene products.
Article in Journal of virology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 51 citations in OpenAlex.
- Critical Role for the Human Cytomegalovirus Major Immediate Early Proteins in Recruitment of RNA Polymerase II and H3K27Ac To an Enhancer-Like Element in OriMicrobiology spectrum · 2023Article
- Human Cytomegalovirus Primary Infection and Reactivation: Insights From Virion-Carried Molecules.Frontiers in microbiology · 2020Review
- Baculovirus as an efficient vector for gene delivery into mosquitoes.Scientific reports · 2018Article
- The 6-Aminoquinolone WC5 inhibits different functions of the immediate-early 2 (IE2) protein of human cytomegalovirus that are essential for viral replication.Antimicrobial agents and chemotherapy · 2014Article
- BclAF1 restriction factor is neutralized by proteasomal degradation and microRNA repression during human cytomegalovirus infection.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The intracellular DNA sensor IFI16 gene acts as restriction factor for human cytomegalovirus replication.PLoS pathogens · 2012Article
- Functional properties of the human cytomegalovirus IE86 protein required for transcriptional regulation and virus replication.Journal of virology · 2010Article
- Article
- Prolonged activation of NF-kappaB by human cytomegalovirus promotes efficient viral replication and late gene expression.Virology · 2006Article
- The phosphorylation status of the serine-rich region of the human cytomegalovirus 86-kilodalton major immediate-early protein IE2/IEP86 affects temporal viral gene expression.Journal of virology · 2005Article
- Analysis of splice variants of the immediate-early 1 region of human cytomegalovirus.Journal of virology · 2004Article
- Ability of the human cytomegalovirus IE1 protein to modulate sumoylation of PML correlates with its functional activities in transcriptional regulation and infectivity in cultured fibroblast cells.Journal of virology · 2004Article
- Phosphorylation of human cytomegalovirus glycoprotein B (gB) at the acidic cluster casein kinase 2 site (Ser900) is required for localization of gB to the trans-Golgi network and efficient virus replication.Journal of virology · 2004Article
- Strain variations in single amino acids of the 86-kilodalton human cytomegalovirus major immediate-early protein (IE2) affect its functional and biochemical properties: implications of dynamic protein conformation.Journal of virology · 2003Article
- Absence of IE1 p72 protein function during low-multiplicity infection by human cytomegalovirus results in a broad block to viral delayed-early gene expression.Journal of virology · 2002Article
- Novel immediate-early protein IE19 of human cytomegalovirus activates the origin recognition complex I promoter in a cooperative manner with IE72.Journal of virology · 2002Article
- Construction of a rationally designed human cytomegalovirus variant encoding a temperature-sensitive immediate-early 2 protein.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Characterization of a human cytomegalovirus with phosphorylation site mutations in the immediate-early 2 protein.Journal of virology · 2002Article
- Dominance of virus over host factors in cross-species activation of human cytomegalovirus early gene expression.Journal of virology · 2001Article
- Activation of transcription of the human cytomegalovirus early UL4 promoter by the Ets transcription factor binding element.Journal of virology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To determine the mechanisms involved in the regulation of human cytomegalovirus early gene expression, we have examined the gene that encodes the viral DNA polymerase (UL54, pol). Our previous studies demonstrated that sequences required for activation of the pol promoter by immediate-early proteins are contained within a region from -128 to +20 and that cellular proteins can bind to this activation domain. In this study, we demonstrate by competition analysis that binding of cellular proteins to pol is associated with an 18-bp region containing a single copy of a novel inverted repeat, IR1. Time course analysis indicated that viral infection increased the level of protein binding to IR1, concurrent with the activation of the pol promoter. Mutation of the IR1 element abrogated binding of cellular factors to the pol promoter and reduced by threefold the activation by immediate-early proteins. Similarly, mutation of IR1 rendered the promoter poorly responsive to activation by viral infection. Mutation of additional sequence elements in the pol promoter had little effect, indicating that IR1 plays the major role in pol promoter regulation. These studies demonstrate that the interaction between cellular factors and IR1 is important for the regulation of expression of the polymerase gene by viral proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.