ArticleThe Journal of cell biology1994
Sense and antisense modification of glial alpha B-crystallin production results in alterations of stress fiber formation and thermoresistance.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 96 citations in OpenAlex.
- HSPB7 interacts with dimerized FLNC and its absence results in progressive myopathy in skeletal muscles.Journal of cell science · 2016Article
- Small Heat Shock Protein αB-Crystallin Controls Shape and Adhesion of Glioma and Myoblast Cells in the Absence of Stress.PloS one · 2016Article
- Abnormally upregulated αB-crystallin was highly coincidental with the astrogliosis in the brains of scrapie-infected hamsters and human patients with prion diseases.Journal of molecular neuroscience : MN · 2013Article
- Different anti-aggregation and pro-degradative functions of the members of the mammalian sHSP family in neurological disorders.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Strategies for treatment in Alexander disease.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2010Review
- Oxygen and blood flow: players in the pathogenesis of glaucoma.Molecular vision · 2008Review
- GFAP and its role in Alexander disease.Experimental cell research · 2007Review
- Heat shock protein 27: its potential role in vascular disease.International journal of experimental pathology · 2006Review
- Co-induction of alphaB-crystallin and MAPKAPK-2 in astrocytes in the penumbra after transient focal cerebral ischemia.Experimental brain research · 2005Article
- The distribution of heat shock proteins in the nervous system of the unstressed mouse embryo suggests a role in neuronal and non-neuronal differentiation.Cell stress & chaperones · 2000Article
- Formation of GFAP cytoplasmic inclusions in astrocytes and their disaggregation by alphaB-crystallin.The American journal of pathology · 1999Article
- MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase.The Journal of cell biology · 1998Article
- Growth and differentiation of C2 myogenic cells are dependent on serum response factor.Molecular and cellular biology · 1996Article
- alpha-Crystallins, versatile stress-proteins.Molecular biology reports · 1995Review
- Modulation of cellular thermoresistance and actin filament stability accompanies phosphorylation-induced changes in the oligomeric structure of heat shock protein 27.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The phenotypic effects of selectively altering the levels of alpha B-crystallin in cultured glial cells were analyzed using sense and antisense approaches. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat alpha B-crystallin sense cDNA or an antisense cDNA regulated by a Rous sarcoma virus promoter to alter cellular levels of alpha B-crystallin. The antisense strategy resulted in decreased alpha B-crystallin levels, as revealed by Western blot and immunocytochemical analyses. The reduced alpha B-crystallin expression was accompanied by alterations in cellular phenotype: (a) a reduction of cell size and/or a slender cell morphology; (b) a disorganized microfilament network; and (c) a reduction of cell adhesiveness. Like HSP27, the presence of additional alpha B-crystallin protein confers a thermoresistant phenotype to stable transfectants. Thus, alpha B-crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization.
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