ArticleThe Journal of cell biology1994
The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed, 225 citations in OpenAlex.
- Cancer-associated exportin-6 upregulation inhibits the transcriptionally repressive and anticancer effects of nuclear profilin-1.Cell reports · 2021Article
- SerFrontiers in cell and developmental biology · 2021Article
- Article
- Genetic suppression of defective profilin by attenuated Myosin II reveals a potential role for Myosin II in actin dynamics in vivo in fission yeast.Molecular biology of the cell · 2020Article
- Splicing Defects of the Profilin Gene Alter Actin Dynamics in an S. pombe SMN Mutant.iScience · 2020Article
- Internetwork competition for monomers governs actin cytoskeleton organization.Nature reviews. Molecular cell biology · 2016Article
- An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System.Genetics · 2015Review
- Subcellular localization and Ser-137 phosphorylation regulate tumor-suppressive activity of profilin-1.The Journal of biological chemistry · 2015Article
- Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12.Molecular biology of the cell · 2015Article
- Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.Developmental cell · 2015Article
- Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways.Current biology : CB · 2013Article
- Overexpression of profilin 3 affects cell elongation and F-actin organization in Arabidopsis thaliana.Plant cell reports · 2013Article
- Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast.The Journal of cell biology · 2012Article
- Anterior PAR proteins function during cytokinesis and maintain DYN-1 at the cleavage furrow in Caenorhabditis elegans.Cytoskeleton (Hoboken, N.J.) · 2012Article
- Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives.Cytoskeleton (Hoboken, N.J.) · 2012Review
- Fission yeast Ags1 confers the essential septum strength needed for safe gradual cell abscission.The Journal of cell biology · 2012Article
- NAC1 is an actin-binding protein that is essential for effective cytokinesis in cancer cells.Cancer research · 2012Article
- Myosin concentration underlies cell size-dependent scalability of actomyosin ring constriction.The Journal of cell biology · 2011Article
- Crosstalk between NDR kinase pathways coordinates cell cycle dependent actin rearrangements.Cell division · 2011Article
- Piccolo regulates the dynamic assembly of presynaptic F-actin.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
40 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The fission yeast Schizosaccharomyces pombe divides by medial fission and, like many higher eukaryotic cells, requires the function of an F-actin contractile ring for cytokinesis. In S. pombe, a class of cdc- mutants defective for cytokinesis, but not for DNA replication, mitosis, or septum synthesis, have been identified. In this paper, we present the characterization of one of these mutants, cdc3-124. Temperature shift experiments reveal that mutants in cdc3 are incapable of forming an F-actin contractile ring. We have molecularly cloned cdc3 and used the cdc3+ genomic DNA to create a strain carrying a cdc3 null mutation by homologous recombination in vivo. Cells bearing a cdc3-null allele are inviable. They arrest the cell cycle at cytokinesis without forming a contractile ring. DNA sequence analysis of the cdc3+ gene reveals that it encodes profilin, an actin-monomer-binding protein. In light of recent studies with profilins, we propose that Cdc3-profilin plays an essential role in cytokinesis by catalyzing the formation of the F-actin contractile ring. Consistent with this proposal are our observations that Cdc3-profilin localizes to the medial region of the cell where the F-actin contractile ring forms, and that it is essential for F-actin ring formation. Cells overproducing Cdc3-profilin become elongated, dumbbell shaped, and arrest at cytokinesis without any detectable F-actin staining. This effect of Cdc3-profilin overproduction is relieved by introduction of a multicopy plasmid carrying the actin encoding gene, act1+. We attribute these effects to potential sequestration of actin monomers by profilin, when present in excess.
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