ArticleThe Journal of cell biology1994
Compartmentalized structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.
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Who cites it
120 citing papers in PubMed, 269 citations in OpenAlex.
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- Multiscale Modelling, Analysis and Simulation of Cancer Invasion Mediated by Bound and Soluble Enzymes.Bulletin of mathematical biology · 2025Article
- Correlative single-molecule and structured illumination microscopy of fast dynamics at the plasma membrane.Nature communications · 2024Article
- Confinement energy landscape classification reveals membrane receptor nano-organization mechanisms.Biophysical journal · 2024Article
- Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane.Frontiers in molecular biosciences · 2024Review
- Leaflet Tensions Control the Spatio-Temporal Remodeling of Lipid Bilayers and Nanovesicles.Biomolecules · 2023Review
- Evolution of the Concepts of Architecture and Supramolecular Dynamics of the Plasma Membrane.Membranes · 2023Review
- Purification Analysis, Intracellular Tracking, and Colocalization of Extracellular Vesicles Using Atomic Force and 3D Single-Molecule Localization Microscopy.Analytical chemistry · 2023Article
- Spatially Resolved Proteomic Analysis of the Lens Extracellular Diffusion Barrier.Investigative ophthalmology & visual science · 2021Article
- Nanopore-mediated protein delivery enabling three-color single-molecule tracking in living cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/γ-secretase at the cell surface.eLife · 2020Article
- Principles and Applications of Biological Membrane Organization.Annual review of biophysics · 2020Review
- Increased Confinement and Polydispersity of STIM1 and Orai1 after CaBiophysical journal · 2020Article
- Lipid Dynamics in Membranes Slowed Down by Transmembrane Proteins.Frontiers in cell and developmental biology · 2020Article
- Do ABC transporters regulate plasma membrane organization?Cellular & molecular biology letters · 2020Review
- Time-Resolved Laurdan Fluorescence Reveals Insights into Membrane Viscosity and Hydration Levels.Biophysical journal · 2018Article
- Numerical Simulation and FRAP Experiments Show That the Plasma Membrane Binding Protein PH-EFA6 Does Not Exhibit Anomalous Subdiffusion in Cells.Biomolecules · 2018Article
- Functional organization of postsynaptic glutamate receptors.Molecular and cellular neurosciences · 2018Review
- SPT and Imaging FCS Provide Complementary Information on the Dynamics of Plasma Membrane Molecules.Biophysical journal · 2018Article
- Electrophoretic cytometry of adherent cells.Lab on a chip · 2017Article
60 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Movements of transferrin and alpha 2-macroglobulin receptor molecules in the plasma membrane of cultured normal rat kidney (NRK) fibroblastic cells were investigated by video-enhanced contrast optical microscopy with 1.8 nm spatial precision and 33 ms temporal resolution by labeling the receptors with the ligand-coated nanometer-sized colloidal gold particles. For both receptor species, most of the movement trajectories are of the confined diffusion type, within domains of approximately 0.25 microns2 (500-700 nm in diagonal length). Movement within the domains is random with a diffusion coefficient approximately 10(-9) cm2/s, which is consistent with that expected for free Brownian diffusion of proteins in the plasma membrane. The receptor molecules move from one domain to one of the adjacent domains at an average frequency of 0.034 s-1 (the residence time within a domain approximately 29 s), indicating that the plasma membrane is compartmentalized for diffusion of membrane receptors and that long-range diffusion is the result of successive intercompartmental jumps. The macroscopic diffusion coefficients for these two receptor molecules calculated on the basis of the compartment size and the intercompartmental jump rate are approximately 2.4 x 10(-11) cm2/s, which is consistent with those determined by averaging the long-term movements of many particles. Partial destruction of the cytoskeleton decreased the confined diffusion mode, increased the simple diffusion mode, and induced the directed diffusion (transport) mode. These results suggest that the boundaries between compartments are made of dynamically fluctuating membrane skeletons (membrane-skeleton fence model).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.