ArticleJournal of bacteriology1994
Isolation and characterization of the Methylophilus sp. strain DM11 gene encoding dichloromethane dehalogenase/glutathione S-transferase.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 69 citations in OpenAlex.
- Genome-Wide Transcription Start Sites Mapping inMicroorganisms · 2022Article
- Characterization of affinity-purified isoforms of Acinetobacter calcoaceticus Y1 glutathione transferases.TheScientificWorldJournal · 2014Article
- Low-scale expression and purification of an active putative iduronate 2-sulfate sulfatase-Like enzyme from Escherichia coli K12.Journal of microbiology (Seoul, Korea) · 2013Article
- Crystallization and preliminary X-ray diffraction analysis of a glutathione S-transferase from Xylella fastidiosa.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- DNA polymerase I is essential for growth of Methylobacterium dichloromethanicum DM4 with dichloromethane.Journal of bacteriology · 2000Article
- Modulation of the glutathione S-transferase in Ochrobactrum anthropi: function of xenobiotic substrates and other forms of stress.The Biochemical journal · 2000Article
- Expression of two glutathione S-transferase genes in the yeast Issatchenkia orientalis is induced by o-dinitrobenzene during cell growth arrest.Journal of bacteriology · 1999Article
- Molecular cloning, expression and site-directed mutagenesis of glutathione S-transferase from Ochrobactrum anthropi.The Biochemical journal · 1998Article
- Effects of bacterial host and dichloromethane dehalogenase on the competitiveness of methylotrophic bacteria growing with dichloromethane.Applied and environmental microbiology · 1998Article
- The atrazine catabolism genes atzABC are widespread and highly conserved.Journal of bacteriology · 1998Article
- Bacterial glutathione S-transferases: what are they good for?Journal of bacteriology · 1997Review
- Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag.Protein science : a publication of the Protein Society · 1997Article
- Molecular cloning and overexpression of a glutathione transferase gene from Proteus mirabilis.The Biochemical journal · 1996Article
- Bacterial growth with chlorinated methanes.Environmental health perspectives · 1995Review
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The restricted facultative methylotroph Methylophilus sp. strain DM11 utilizes dichloromethane as the sole carbon and energy source. It differs from other dichloromethane-utilizing methylotrophs by faster growth on this substrate and by possession of a group B dichloromethane dehalogenase catalyzing dechlorination at a fivefold-higher rate than the group A enzymes of slow-growing strains. We isolated dcmA, the structural gene of the strain DM11 dichloromethane dehalogenase, to elucidate its relationship to the previously characterized dcmA gene of Methylobacterium sp. strain DM4, which encodes a group A enzyme. Nucleotide sequence determination of dcmA from strain DM11 predicts a protein of 267 amino acids, corresponding to a molecular mass of 31,197 Da. The 5' terminus of in vivo dcmA transcripts was determined by primer extension to be 70 bp upstream of the translation initiation codon. It was preceded by a putative promoter sequence with high resemblance to the Escherichia coli sigma 70 consensus promoter sequence. dcmA and 130 bp of its upstream sequence were brought under control of the tac promoter and expressed in E. coli to approximately 20% of the total cellular protein by induction with isopropylthiogalactopyranoside (IPTG) and growth at 25 degrees C. Expression at 37 degrees C led to massive formation of inclusion bodies. Comparison of the strain DM11 and strain DM4 dichloromethane dehalogenase sequences revealed 59% identity at the DNA level and 56% identity at the protein level, thus indicating an ancient divergence of the two enzymes. Both dehalogenases are more closely related to eukaryotic class theta glutathione S-transferases than to a number of bacterial glutathione S-transferases.
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