ArticlePlant molecular biology1994
Molecular analysis of wild-type and mutant alleles at the Opaque-2 regulatory locus of maize reveals different mutations and types of O2 products.
Article in Plant molecular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Designed Alleles of ZmRap2.7 Decouple the Trade-Off Between Early Flowering and Yield Penalty.Plant biotechnology journal · 2026Article
- ZmICE1a regulates the defence-storage trade-off in maize endosperm.Nature plants · 2024Article
- Nitrogen-dependent binding of the transcription factor PBF1 contributes to the balance of protein and carbohydrate storage in maize endosperm.The Plant cell · 2023Article
- The Italian Research on the Molecular Characterization of Maize Kernel Development.International journal of molecular sciences · 2022Review
- Article
- A SnRK1-The Plant cell · 2020Article
- Maize Dek33 encodes a pyrimidine reductase in riboflavin biosynthesis that is essential for oil-body formation and ABA biosynthesis during seed development.Journal of experimental botany · 2019Article
- SEED CAROTENOID DEFICIENT Functions in Isoprenoid Biosynthesis via the Plastid MEP Pathway.Plant physiology · 2019Article
- The ZmbZIP22 Transcription Factor Regulates 27-kD γ-Zein Gene Transcription during Maize Endosperm Development.The Plant cell · 2018Article
- Opaque-2 Regulates a Complex Gene Network Associated with Cell Differentiation and Storage Functions of Maize Endosperm.The Plant cell · 2018Article
- OPAQUE11 Is a Central Hub of the Regulatory Network for Maize Endosperm Development and Nutrient Metabolism.The Plant cell · 2018Article
- Uniparental and transgressive expression of α-zeins in maize endosperm of o2 hybrid lines.PloS one · 2018Article
- Proline responding1 Plays a Critical Role in Regulating General Protein Synthesis and the Cell Cycle in Maize.The Plant cell · 2014Article
- Opaque7 encodes an acyl-activating enzyme-like protein that affects storage protein synthesis in maize endosperm.Genetics · 2011Article
- Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site.Plant physiology · 2007Article
- Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro.Plant molecular biology · 2001Article
- Specific combinations of zein genes and genetic backgrounds influence the transcription of the heavy-chain zein genes in maize opaque-2 endosperms.Plant physiology · 2000Article
- The activity of the maize Opaque2 transcriptional activator is regulated diurnally.Plant physiology · 1999Article
- Phosphorylation of Opaque2 changes diurnally and impacts its DNA binding activity.The Plant cell · 1997Article
- The involvement of Opaque 2 on beta-prolamin gene regulation in maize and Coix suggests a more general role for this transcriptional activator.Plant molecular biology · 1995Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the various members of the zein multigene family in maize endosperm is controlled by different regulatory loci. One of these loci, Opaque-2, coding for a bZIP transcriptional factor, controls the expression of a subset of zein genes. Analysis of genomic DNA from plants carrying wild-type (O2) or mutant o2 alleles shows specific DNA restriction patterns that correlate with transcript types and their various gene products. Northern and western analyses show the presence in different wild types of a 1.7 kb transcript coding for different sizes of normal O2 proteins that migrate as doublets in the 68-72 kDa range. Among the various o2 mutants analysed we showed the occurrence of various null-transcript alleles, the presence of alleles with a normal size transcript which, however, produce a different-sized o2 protein, and a mutant producing both a normal size transcript and a longer transcript, but generating only a single o2 product migrating around 40 kDa. Analysis of other mutations (o7, fl2) known to affect zein polypeptide synthesis shows no interference of these mutations in the expression of the O2 gene products. The overall results indicate the occurrence of micro heterogeneity in the O2 wild-type genes and a broad spectrum of o2 mutations, both producing different sizes of O2 or o2 proteins. A nomenclature of the O2 and o2 genes based on the RFLP, transcripts and products of the various alleles is presented.
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