ArticleProceedings of the National Academy of Sciences of the United States of America1994
Senescence of immortal human fibroblasts by the introduction of normal human chromosome 6.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 96 citations in OpenAlex.
- Alternative Promoters ofGenes · 2022Article
- Review
- Chromosome 6 encoded RNaseT2 protein is a cell growth regulator.Journal of cellular and molecular medicine · 2010Article
- Significance of cellular senescence in aging and cancer.Cancer research and treatment · 2009Article
- PHF10 is required for cell proliferation in normal and SV40-immortalized human fibroblast cells.Cytogenetic and genome research · 2009Article
- Critical pathways in cellular senescence and immortalization revealed by gene expression profiling.Oncogene · 2008Review
- The manipulation of chromosomes by mankind: the uses of microcell-mediated chromosome transfer.Chromosoma · 2005Review
- Functional identification of a BAC clone from 16q24 carrying a senescence gene SEN16 for breast cancer cells.Oncogene · 2005Article
- Article
- Article
- Functional evidence for a squamous cell carcinoma mortality gene(s) on human chromosome 4.Oncogene · 2002Article
- Telomerase inhibition by peptide nucleic acids reverses 'immortality' of transformed human cells.Oncogene · 1999Article
- Identification of a gene at 16q24.3 that restores cellular senescence in immortal mammary tumor cells.Oncogene · 1999Article
- A novel region of deletion on chromosome 6q23.3 spanning less than 500 Kb in high grade invasive epithelial ovarian cancer.Oncogene · 1999Article
- Mechanism of immortalization.Age · 1999Article
- The genetics of cellular senescence.American journal of human genetics · 1998Review
- Specific chromosomal imbalances in human papillomavirus-transfected cells during progression toward immortality.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Induction of senescence-like phenotypes by forced expression of hic-5, which encodes a novel LIM motif protein, in immortalized human fibroblasts.Molecular and cellular biology · 1997Article
- Restoration of the cellular senescence program and repression of telomerase by human chromosome 3.Japanese journal of cancer research : Gann · 1995Article
- The genetic analysis of ovarian cancer.British journal of cancer · 1995Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In these studies we show that introduction of a normal human chromosome 6 or 6q can suppress the immortal phenotype of simian virus 40-transformed human fibroblasts (SV/HF). Normal human fibroblasts have a limited life span in culture. Immortal clones of SV/HF displayed nonrandom rearrangements in chromosome 6. Single human chromosomes present in mouse/human monochromosomal hybrids were introduced into SV/HF via microcell fusion and maintained by selection for a dominant selectable marker gpt, previously integrated into the human chromosome. Clones of SV/HF cells bearing chromosome 6 displayed limited potential for cell division and morphological characteristics of senescent cells. The loss of chromosome 6 from the suppressed clones correlated with the reappearance of immortal clones. Introduced chromosome 6 in the senescing cells was distinguished from those of parental cells by the analysis for DNA sequences specific for the donor chromosome. Our results further show that suppression of immortal phenotype in SV/HF is specific to chromosome 6. Introduction of individual human chromosomes 2, 8, or 19 did not impart cellular senescence in SV/HF. In addition, introduction of chromosome 6 into human glioblastoma cells did not lead to senescence. Based upon these results we propose that at least one of the genes (SEN6) for cellular senescence in human fibroblasts is present on the long arm of chromosome 6.
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