ArticleNucleic acids research1994
Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- A bacterial artificial chromosome library for sequencing the complete human genome.Genome research · 2001Article
- Null mutation of PCLN-1/Claudin-16 results in bovine chronic interstitial nephritis.Genome research · 2000Article
- Characterization and application of soybean YACs to molecular cytogenetics.Molecular & general genetics : MGG · 1996Article
- Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Physical structure of human chromosome 21: an analysis of YACs spanning 21q.Mammalian genome : official journal of the International Mammalian Genome Society · 1995Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chimerism is a major limitation of current YAC libraries. A method based on partially filled-in ends of restriction fragments was designed to avoid coligation as a possible source of chimeras. Model experiments using plasmid DNA as an insert showed that coligation was clearly avoided by this method. Pilot collections of YACs with an average insert size of 650kb were then constructed with and without the partial fill-in treatment. Starting from a mixture of a equal amounts of human and mouse DNA, none of 108 clones was positive by hybridization with both Alu and B2 probes, again suggesting that coligation was effectively blocked. However, 4 out of 10 clones still hybridized to 2 or more locations by FISH on chromosomes in human metaphase spreads, level similar to that in the clones made without the partial fill-in step. These results strongly suggest that chimeric clones generally arise by a mechanism independent of coligation, presumptively based on recombination.
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