ArticleNucleic acids research1994
Intramolecular signal transduction within the FixJ transcriptional activator: in vitro evidence for the inhibitory effect of the phosphorylatable regulatory domain.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Rosetta design with co-evolutionary information retains protein function.PLoS computational biology · 2021Article
- The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions.BMC microbiology · 2015Article
- Development of quorum-based anti-virulence therapeutics targeting Gram-negative bacterial pathogens.International journal of molecular sciences · 2013Review
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- Dual control of Sinorhizobium meliloti RpoE2 sigma factor activity by two PhyR-type two-component response regulators.Journal of bacteriology · 2010Article
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- Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB.BMC microbiology · 2008Article
- Regulation of the Myxococcus xanthus C-signal-dependent Omega4400 promoter by the essential developmental protein FruA.Journal of bacteriology · 2006Article
- PhoP can activate its target genes in a PhoQ-independent manner.Journal of bacteriology · 2004Article
- ADP reduces the oxygen-binding affinity of a sensory histidine kinase, FixL: the possibility of an enhanced reciprocating kinase reaction.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- N-acyl homoserine lactone binding to the CarR receptor determines quorum-sensing specificity in Erwinia.The EMBO journal · 2000Article
- Modulation of NifA activity by PII in Azospirillum brasilense: evidence for a regulatory role of the NifA N-terminal domain.Journal of bacteriology · 1996Article
- Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes.Journal of bacteriology · 1996Article
- Structural and functional analysis of the fixLJ genes of Rhizobium leguminosarum biovar phaseoli CNPAF512.Molecular & general genetics : MGG · 1995Article
- The amino-terminal domain of the prokaryotic enhancer-binding protein XylR is a specific intramolecular repressor.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
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5 authors.
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Abstract
FixJ is a phosphorylatable 'response regulator' controlling the transcription of the key nitrogen fixation genes nifA and fixK in Rhizobium meliloti. Sequence and genetic analyses indicated that FixJ comprises an N-terminal phosphorylatable regulatory domain, FixJN, and a C-terminal transcriptional activator domain, FixJC. We have now overexpressed and purified the FixJC protein and show that it is fully active in an in vitro transcription system with purified RNA polymerase. FixJC appeared to act synergistically with RNA polymerase at the nifA promoter. Furthermore FixJC was more active in vitro than the full-length dephosphorylated FixJ protein. Therefore activity of FixJC is inhibited by FixJN within the FixJ protein. This inhibition is relieved by phosphorylation of FixJN. Such a negative mode of intramolecular signal transduction may be generalizable to other response regulators.
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