ArticleThe Journal of clinical investigation1994
Inhibition of bone resorption in vitro by antisense RNA and DNA molecules targeted against carbonic anhydrase II or two subunits of vacuolar H(+)-ATPase.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 100 citations in OpenAlex.
- The pathogenesis and intersecting mechanisms of monogenic bone mass disorders.Nature reviews. Endocrinology · 2026Review
- Hidden targets in dermatology:Journal of enzyme inhibition and medicinal chemistry · 2025Article
- Osteoporosis in Asthma and COPD.Indian journal of orthopaedics · 2023Review
- Quantitative Trait Locus and Integrative Genomics Revealed Candidate Modifier Genes for Ectopic Mineralization in Mouse Models of Pseudoxanthoma Elasticum.The Journal of investigative dermatology · 2019Article
- Dynamic mRNA Expression Analysis of the Secondary Palatal Morphogenesis in Miniature Pigs.International journal of molecular sciences · 2019Article
- Potential oligogenic disease of mental retardation, short stature, spastic paraparesis, and osteopetrosis.The application of clinical genetics · 2018Article
- Spatial regulation of controlled bioactive factor delivery for bone tissue engineering.Advanced drug delivery reviews · 2015Review
- Osteoporosis in chronic obstructive pulmonary disease.Clinical medicine insights. Circulatory, respiratory and pulmonary medicine · 2015Review
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- Article
- Versatile roles of V-ATPases accessory subunit Ac45 in osteoclast formation and function.PloS one · 2011Article
- Proteomic analysis of osteoclast lipid rafts: the role of the integrity of lipid rafts on V-ATPase activity in osteoclasts.Journal of bone and mineral metabolism · 2010Article
- Advances in osteoclast biology resulting from the study of osteopetrotic mutations.Human genetics · 2009Review
- Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems.Journal of negative results in biomedicine · 2005Article
- A novel inhibitor of vacuolar ATPase, FR167356, which can discriminate between osteoclast vacuolar ATPase and lysosomal vacuolar ATPase.British journal of pharmacology · 2004Article
- A selective inhibitor of the osteoclastic V-H(+)-ATPase prevents bone loss in both thyroparathyroidectomized and ovariectomized rats.The Journal of clinical investigation · 2000Article
- Molecular aspects of osteoclast function.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 1996Review
- Resorption-cycle-dependent polarization of mRNAs for different subunits of V-ATPase in bone-resorbing osteoclasts.Molecular biology of the cell · 1996Article
- Acid extrusion is induced by osteoclast attachment to bone. Inhibition by alendronate and calcitonin.The Journal of clinical investigation · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bone resorbing cells, osteoclasts, express high levels of carbonic anhydrase II (CA II) and vacuolar H(+)-ATPase (V-ATPase) during bone resorption. We have used antisense RNA and DNA molecules targeted against CA II, and against 16- and 60-kD subunits of vacuolar H(+)-ATPase (V-ATPase), to block the expression of these proteins in vitro. Osteoclastic bone resorption was studied in two in vitro culture systems: release of 45Calcium from prelabeled newborn mouse calvaria cultures, and resorption pit assays performed with rat osteoclasts cultured on bovine bone slices. Both antisense RNA and DNA against CA II and the V-ATPase were used to compare their specificities as regards inhibiting bone resorption in vitro. The antisense molecules inhibited the synthesis of these proteins by decreasing the amounts of mRNA in the cells in a highly specific manner. In osteoclast cultures treated with the 16-kD V-ATPase antisense RNA, acidification of an unknown population of intracellular vesicles was highly stimulated. The acidification of these vesicles was not sensitive to amiloride or bafilomycin A1. This suggests the existence of a back-up system for acidification of intracellular vesicles, when the expression of the V-ATPase is blocked. Our results further indicate that blocking the expression of CA II and V-ATPase with antisense RNA or DNA leads to decreased bone resorption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.