ArticleMolecular and cellular biology1994
Serum amyloid A gene expression under acute-phase conditions involves participation of inducible C/EBP-beta and C/EBP-delta and their activation by phosphorylation.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 118 citations in OpenAlex.
- Basic biology and roles of CEBPD in cardiovascular disease.Cell death discovery · 2025Review
- Kidney Involvement in Autoinflammatory Diseases.Kidney diseases (Basel, Switzerland) · 2023Review
- Article
- Hepatocytes: a key cell type for innate immunity.Cellular & molecular immunology · 2016Review
- STAT3 Establishes an Immunosuppressive Microenvironment during the Early Stages of Breast Carcinogenesis to Promote Tumor Growth and Metastasis.Cancer research · 2016Article
- Serum Amyloid A Protein Concentration in Blood is Influenced by Genetic Differences in the Cheetah (Acinonyx jubatus).The Journal of heredity · 2016Article
- Biological roles of CCAAT/Enhancer-binding protein delta during inflammation.Journal of biomedical science · 2015Article
- C/EBP transcription factors regulate NADPH oxidase in human aortic smooth muscle cells.Journal of cellular and molecular medicine · 2014Article
- Novel RNA oligonucleotide improves liver function and inhibits liver carcinogenesis in vivo.Hepatology (Baltimore, Md.) · 2014Article
- Identification of p130Cas/ErbB2-dependent invasive signatures in transformed mammary epithelial cells.Cell cycle (Georgetown, Tex.) · 2013Article
- The many faces of C/EBPδ and their relevance for inflammation and cancer.International journal of biological sciences · 2013Review
- CCAAT/Enhancer Binding Protein-delta (C/EBP-delta) regulates cell growth, migration and differentiation.Cancer cell international · 2010Article
- Requirement for nuclear factor kappa B signalling in the interleukin-1-induced expression of the CCAAT/enhancer binding protein-delta gene in hepatocytes.The international journal of biochemistry & cell biology · 2010Article
- Identification of a Stat3-dependent transcription regulatory network involved in metastatic progression.Cancer research · 2009Article
- O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation.The Journal of biological chemistry · 2009Article
- Identification and characterization of CCAAT/Enhancer Binding proteindelta (C/EBPdelta) target genes in G0 growth arrested mammary epithelial cells.BMC molecular biology · 2008Article
- Transcriptional regulation of cyclooxygenase-2 in response to proteasome inhibitors involves reactive oxygen species-mediated signaling pathway and recruitment of CCAAT/enhancer-binding protein delta and CREB-binding protein.Molecular biology of the cell · 2005Article
- Orthogonal analysis of C/EBPbeta targets in vivo during liver proliferation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Hepatocyte nuclear factor 4 response to injury involves a rapid decrease in DNA binding and transactivation via a JAK2 signal transduction pathway.The Biochemical journal · 2002Article
- CCAAT/enhancer-binding proteins: structure, function and regulation.The Biochemical journal · 2002Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Serum amyloid A (SAA) is a plasma protein whose synthesis is markedly increased in the liver during the inflammatory process. Previous analysis of SAA promoter function implicated the involvement of the CCAAT/enhancer-binding protein (C/EBP) in controlling this process. In this study, using antibodies against three C/EBP isoforms in DNA-binding and Western blot (immunoblot) assays, we found that in response to inflammatory signals, both C/EBP-delta and C/EBP-beta are induced and that their interactions with the SAA promoter element are necessary for the increased SAA gene expression. Cotransfections of liver cells with an SAA promoter-linked reporter chloramphenicol acetyltransferase gene and murine sarcoma virus-expressed C/EBP-delta or C/EBP-beta confirm such phenomena. The increased transactivating ability in the presence of the cellular phosphatase inhibitors okadaic acid and sodium orthovanadate, coupled with the observation that dephosphorylation severely inhibits the DNA-binding ability in vitro, implicates a role of phosphorylation in the regulation of the activities of the C/EBP-delta isoform. Consistent with these findings, we have detected higher levels of DNA-binding activity of C/EBP-delta prepared from cells treated with phosphatase inhibitors. We also present evidence that C/EBP-delta is a phosphoprotein. These results suggest that C/EBP-delta is regulated by phosphorylation and, in conjunction with C/EBP-beta, is one of the major proteins responsible for the increased transcription of the SAA gene in response to inflammatory stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.