ArticleMolecular and cellular biology1994
Effects of mutant Ran/TC4 proteins on cell cycle progression.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- GAP Activity, but Not Subcellular Targeting, Is Required for Arabidopsis RanGAP Cellular and Developmental Functions.The Plant cell · 2015Article
- Increased cathepsin D protein expression is a biomarker for osteosarcomas, pulmonary metastases and other bone malignancies.Oncotarget · 2015Article
- Characterization of a novel activated Ran GTPase mutant and its ability to induce cellular transformation.The Journal of biological chemistry · 2012Article
- Activation of the Ran GTPase is subject to growth factor regulation and can give rise to cellular transformation.The Journal of biological chemistry · 2010Article
- Article
- Roles for the E4 orf6, orf3, and E1B 55-kilodalton proteins in cell cycle-independent adenovirus replication.Journal of virology · 1999Article
- E1B 55-kilodalton-associated protein: a cellular protein with RNA-binding activity implicated in nucleocytoplasmic transport of adenovirus and cellular mRNAs.Journal of virology · 1998Article
- The balance of RanBP1 and RCC1 is critical for nuclear assembly and nuclear transport.Molecular biology of the cell · 1997Article
- Nucleocytoplasmic transport of macromolecules.Microbiology and molecular biology reviews : MMBR · 1997Review
- The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle.Journal of virology · 1997Article
- A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1.The Journal of cell biology · 1996Article
- Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import.The Journal of cell biology · 1996Article
- Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine/threonine protein kinase.Molecular & general genetics : MGG · 1996Article
- Mutants in a yeast Ran binding protein are defective in nuclear transport.The EMBO journal · 1995Article
- RanBP1, a Ras-like nuclear G protein binding to Ran/TC4, inhibits RCC1 via Ran/TC4.Molecular & general genetics : MGG · 1995Article
- The Ran/TC4 GTPase-binding domain: identification by expression cloning and characterization of a conserved sequence motif.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.Molecular and cellular biology · 1995Article
- Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1.The EMBO journal · 1995Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Ran/TC4, a member of the RAS gene superfamily, encodes an abundant nuclear protein that binds and hydrolyzes GTP. Transient expression of a Ran/TC4 mutant protein deficient in GTP hydrolysis blocked DNA replication, suggesting a role for Ran/TC4 in the regulation of cell cycle progression. To test this possibility, we exploited an efficient transfection system, involving the introduction of cDNAs in the pMT2 vector into 293/Tag cells, to analyze phenotypes associated with mutant and wild-type Ran/TC4 expression. Expression of a Ran/TC4 mutant protein deficient in GTP hydrolysis inhibited proliferation of transfected cells by arresting them predominantly in the G2, but also in the G1, phase of the cell cycle. Deletion of an acidic carboxy-terminal hexapeptide from the Ran/TC4 mutant did not alter its nuclear localization but did block its inhibitory effect on cell cycle progression. These data suggest that normal progression of the cell cycle is coupled to the operation of a Ran/TC4 GTPase cycle. Mediators of this coupling are likely to include the nuclear regulator of chromosome condensation 1 protein and the mitosis-promoting factor complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.