ArticleMolecular and cellular biology1994
Mapping and mutagenesis of the amino-terminal transcriptional repression domain of the Drosophila Krüppel protein.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Modeling of gap gene expression in Drosophila Kruppel mutants.PLoS computational biology · 2012Article
- FOXE1 polyalanine tract length screening by MLPA in idiopathic premature ovarian failure.Reproductive biology and endocrinology : RB&E · 2011Article
- The gap gene network.Cellular and molecular life sciences : CMLS · 2011Review
- Genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators in Arabidopsis.Plant physiology · 2010Article
- EVOPRINTER, a multigenomic comparative tool for rapid identification of functionally important DNA.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- CtBP-independent repression in the Drosophila embryo.Molecular and cellular biology · 2003Article
- The yeast protein Xtc1 functions as a direct transcriptional repressor.Nucleic acids research · 2002Article
- Cell signaling switches HOX-PBX complexes from repressors to activators of transcription mediated by histone deacetylases and histone acetyltransferases.Molecular and cellular biology · 2000Article
- Expression and function of the homeodomain-containing protein Hex in thyroid cells.Nucleic acids research · 2000Article
- Transcription repression by Xenopus ET and its human ortholog TBX3, a gene involved in ulnar-mammary syndrome.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Adjacent proline residues in the inhibitory domain of the Oct-2 transcription factor play distinct functional roles.Nucleic acids research · 1998Article
- Rex-1, a gene encoding a transcription factor expressed in the early embryo, is regulated via Oct-3/4 and Oct-6 binding to an octamer site and a novel protein, Rox-1, binding to an adjacent site.Molecular and cellular biology · 1998Article
- Involvement of negative cofactor NC2 in active repression by zinc finger-homeodomain transcription factor AREB6.Molecular and cellular biology · 1998Article
- Groucho acts as a corepressor for a subset of negative regulators, including Hairy and Engrailed.Genes & development · 1997Article
- Two evolutionarily conserved repression domains in the Drosophila Kruppel protein differ in activator specificity.Molecular and cellular biology · 1997Article
- Synpolydactyly phenotypes correlate with size of expansions in HOXD13 polyalanine tract.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Net (ERP/SAP2) one of the Ras-inducible TCFs, has a novel inhibitory domain with resemblance to the helix-loop-helix motif.The EMBO journal · 1996Article
- The Gfi-1 proto-oncoprotein contains a novel transcriptional repressor domain, SNAG, and inhibits G1 arrest induced by interleukin-2 withdrawal.Molecular and cellular biology · 1996Article
- The human cut homeodomain protein can repress gene expression by two distinct mechanisms: active repression and competition for binding site occupancy.Molecular and cellular biology · 1996Article
- A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains.The EMBO journal · 1996Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We previously demonstrated that the Drosophila Krüppel protein is a transcriptional repressor with separable DNA-binding and transcriptional repression activities. In this study, the minimal amino (N)-terminal repression region of the Krüppel protein was defined by transferring regions of the Krüppel protein to a heterologous DNA-binding protein, the lacI protein. Fusion of a predicted alpha-helical region from amino acids 62 to 92 in the N terminus of the Krüppel protein was sufficient to transfer repression activity. This putative alpha-helix has several hydrophobic surfaces, as well as a glutamine-rich surface. Mutants containing multiple amino acid substitutions of the glutamine residues demonstrated that this putative alpha-helical region is essential for repression activity of a Krüppel protein containing the entire N-terminal and DNA-binding regions. Furthermore, one point mutant with only a single glutamine on this surface altered to lysine abolished the ability of the Krüppel protein to repress, indicating the importance of the amino acid at residue 86 for repression. The N terminus also contained an adjacent activation region localized between amino acids 86 and 117. Finally, in accordance with predictions from primary amino acid sequence similarity, a repression region from the Drosophila even-skipped protein, which was six times more potent than that of the Krüppel protein in the mammalian cells, was characterized. This segment included a hydrophobic stretch of 11 consecutive alanine residues and a proline-rich region.
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