ArticleMolecular and cellular biology1994
The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
67 citing papers in PubMed, 144 citations in OpenAlex.
- Current Nuclear Engineering Strategies in the Green MicroalgaLife (Basel, Switzerland) · 2023Review
- Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas.Plant physiology · 2021Article
- Good News for Nuclear Transgene Expression inCells · 2019Review
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia.Journal of cell science · 2019Article
- Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise inPlant physiology · 2017Article
- Current status and perspectives of genome editing technology for microalgae.Biotechnology for biofuels · 2017Review
- Knock-Down of the IFR1 Protein Perturbs the Homeostasis of Reactive Electrophile Species and Boosts Photosynthetic Hydrogen Production inFrontiers in plant science · 2017Article
- A new class of cyclin dependent kinase in Chlamydomonas is required for coupling cell size to cell division.eLife · 2016Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Article
- Purple chromoprotein gene serves as a new selection marker for transgenesis of the microalga Nannochloropsis oculata.PloS one · 2015Article
- Article
- Stable nuclear transformation of Pandorina morum.BMC biotechnology · 2014Article
- Article
- The nexin-dynein regulatory complex subunit DRC1 is essential for motile cilia function in algae and humans.Nature genetics · 2013Article
- CDKL5 regulates flagellar length and localizes to the base of the flagella in Chlamydomonas.Molecular biology of the cell · 2013Article
- Stable nuclear transformation of Eudorina elegans.BMC biotechnology · 2013Article
- Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives.Plant cell reports · 2012Review
- Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency.Cytoskeleton (Hoboken, N.J.) · 2012Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant to the eukaryotic translational inhibitors cryptopleurine and emetine in which the resistance was due to a missense mutation (CRY1-1) in the CRY1 gene; resistance was dominant in heterozygous stable diploids. Cotransformation experiments using the CRY1-1 gene and the gene for nitrate reductase (NIT1) produced a low level of resistance to cryptopleurine and emetine. Resistance levels were increased when the CRY1-1 gene was placed under the control of a constitutive promoter from the ribulose bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) gene. We also found that the 5' untranslated region of the CRY1 gene was required for expression of the CRY1-1 transgene. Direct selection of emetine-resistant transformants was possible when transformed cells were first induced to differentiate into gametes by nitrogen starvation and then allowed to dedifferentiate back to vegetative cells before emetine selection was applied. With this transformation protocol, the RBCS2/CRY1-1 dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.