ArticleMolecular and cellular biology1994
Presence of negative and positive cis-acting RNA splicing elements within and flanking the first tat coding exon of human immunodeficiency virus type 1.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
76 citing papers in PubMed, 130 citations in OpenAlex.
- Review
- Defective HIV-1 genomes and their potential impact on HIV pathogenesis.Retrovirology · 2022Review
- Human Tat-specific factor 1 binds the HIV-1 genome and selectively transports HIV-1 RNAs.Molecular biology reports · 2020Article
- Global synonymous mutagenesis identifies cis-acting RNA elements that regulate HIV-1 splicing and replication.PLoS pathogens · 2018Article
- Characterizing HIV-1 Splicing by Using Next-Generation Sequencing.Journal of virology · 2017Article
- Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication.Retrovirology · 2015Article
- Genomic HEXploring allows landscaping of novel potential splicing regulatory elements.Nucleic acids research · 2014Article
- tat Exon 1 exhibits functional diversity during HIV-1 subtype C primary infection.Journal of virology · 2013Article
- Tra2-mediated recognition of HIV-1 5' splice site D3 as a key factor in the processing of vpr mRNA.Journal of virology · 2013Article
- Article
- Transcriptional and posttranscriptional regulation of HIV-1 gene expression.Cold Spring Harbor perspectives in medicine · 2012Review
- The HIV-1 Tat protein has a versatile role in activating viral transcription.Journal of virology · 2011Article
- Excessive RNA splicing and inhibition of HIV-1 replication induced by modified U1 small nuclear RNAs.Journal of virology · 2010Article
- RNA-binding protein Muscleblind-like 3 (MBNL3) disrupts myocyte enhancer factor 2 (Mef2) {beta}-exon splicing.The Journal of biological chemistry · 2010Article
- Serine- and arginine-rich proteins 55 and 75 (SRp55 and SRp75) induce production of HIV-1 vpr mRNA by inhibiting the 5'-splice site of exon 3.The Journal of biological chemistry · 2010Article
- The transcriptional transactivator Tat selectively regulates viral splicing.Nucleic acids research · 2010Article
- Bipartite functions of the CREB co-activators selectively direct alternative splicing or transcriptional activation.The EMBO journal · 2009Article
- Negative and positive mRNA splicing elements act competitively to regulate human immunodeficiency virus type 1 vif gene expression.Journal of virology · 2008Article
- Insights into the selective activation of alternatively used splice acceptors by the human immunodeficiency virus type-1 bidirectional splicing enhancer.Nucleic acids research · 2008Article
- HIV-1 infection induces changes in expression of cellular splicing factors that regulate alternative viral splicing and virus production in macrophages.Retrovirology · 2008Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The human immunodeficiency virus type 1 (HIV-1) RNA follows a complex splicing pathway in which a single primary transcript either remains unspliced or is alternatively spliced to more than 30 different singly and multiply spliced mRNAs. We have used an in vitro splicing assay to identify cis elements within the viral genome that regulate HIV-1 RNA splicing. A novel splicing regulatory element (SRE) within the first tat coding exon has been detected. This element specifically inhibits splicing at the upstream 3' splice site flanking this tat exon. The element only functions when in the sense orientation and is position dependent when inserted downstream of a heterologous 3' splice site. In vivo, an HIV-1 SRE mutant demonstrated a decrease in unspliced viral RNA, increased levels of single- and double-spliced tat mRNA, and reduced levels of env and rev mRNAs. In addition to the negative cis-acting SRE, the flanking 5' splice site downstream of the first tat coding exon acts positively to increase splicing at the upstream 3' splice sites. These results are consistent with hypotheses of bridging interactions between cellular factors that bind to the 5' splice site and those that bind at the upstream 3' splice site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.