ArticleMolecular and cellular biology1994
Dorsal, a Drosophila Rel-like protein, is phosphorylated upon activation of the transmembrane protein Toll.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 102 citations in OpenAlex.
- Mechanisms for controlling Dorsal nuclear levels.Frontiers in cell and developmental biology · 2024Review
- Specific Isoforms of the Guanine-Nucleotide Exchange Factor dPix Couple Neuromuscular Synapse Growth to Muscle Growth.Developmental cell · 2020Article
- Article
- Calpain A modulates Toll responses by limited Cactus/IκB proteolysis.Molecular biology of the cell · 2013Article
- The IRAK homolog Pelle is the functional counterpart of IκB kinase in the Drosophila Toll pathway.PloS one · 2013Article
- Article
- Spn1 regulates the GNBP3-dependent Toll signaling pathway in Drosophila melanogaster.Molecular and cellular biology · 2011Article
- NF-κB/Rel proteins and the humoral immune responses of Drosophila melanogaster.Current topics in microbiology and immunology · 2011Review
- Article
- WntD is a feedback inhibitor of Dorsal/NF-kappaB in Drosophila development and immunity.Nature · 2005Article
- Regulated assembly of the Toll signaling complex drives Drosophila dorsoventral patterning.The EMBO journal · 2004Article
- The Drosophila nucleoporin DNup88 localizes DNup214 and CRM1 on the nuclear envelope and attenuates NES-mediated nuclear export.The Journal of cell biology · 2003Article
- ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.Nucleic acids research · 2003Article
- DmMyD88 controls dorsoventral patterning of the Drosophila embryo.EMBO reports · 2003Article
- members only encodes a Drosophila nucleoporin required for rel protein import and immune response activation.Genes & development · 2000Article
- A Drosophila ESC-E(Z) protein complex is distinct from other polycomb group complexes and contains covalently modified ESC.Molecular and cellular biology · 2000Article
- Article
- Control of the nuclear localization of Extradenticle by competing nuclear import and export signals.Genes & development · 1999Article
- Nuclear import of the Drosophila Rel protein Dorsal is regulated by phosphorylation.Genes & development · 1999Article
- A multimeric complex and the nuclear targeting of the Drosophila Rel protein Dorsal.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear import of dorsal, a Drosophila Rel homolog, is directed by a spatially restricted extracellular ligand in blastoderm embryos. We have demonstrated both that dorsal is an embryonic phosphoprotein and that its phosphorylation state is regulated by an intracellular signaling pathway initiated by the transmembrane receptor Toll. Immunoblot analysis of cytoplasm from precisely staged embryos revealed that the phosphorylation state of dorsal is altered during the time period that Toll is activated. Moreover, mutations that constitutively activate Toll stimulated dorsal phosphorylation, while mutations that block Toll activation reduced the level of dorsal phosphorylation. We further demonstrated that signal-dependent dorsal phosphorylation is modulated by three intracellular proteins, pelle, tube, and cactus. Using double-mutant embryos, we then explored the nature of the kinase activity responsible for dorsal phosphorylation. We found that free dorsal is a substrate for a signal-independent kinase activity. In addition, our results imply that dorsal is a substrate for a Toll-dependent kinase. These results are consistent with the hypothesis that phosphorylation of Rel-related proteins may be required for the proper nuclear localization and transcriptional activity of these proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.