ArticleThe Journal of cell biology1994
Basolateral protein transport in streptolysin O-permeabilized MDCK cells.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- The many ways to cross the plasma membrane.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Reconstitution of brefeldin A-induced golgi tubulation and fusion with the endoplasmic reticulum in semi-intact chinese hamster ovary cells.Molecular biology of the cell · 2000Article
- Influenza virus assembly and lipid raft microdomains: a role for the cytoplasmic tails of the spike glycoproteins.Journal of virology · 2000Article
- MEK and Cdc2 kinase are sequentially required for Golgi disassembly in MDCK cells by the mitotic Xenopus extracts.The Journal of cell biology · 2000Article
- Induction of caveolae in the apical plasma membrane of Madin-Darby canine kidney cells.The Journal of cell biology · 2000Article
- N-Glycans mediate the apical sorting of a GPI-anchored, raft-associated protein in Madin-Darby canine kidney cells.The Journal of cell biology · 1999Article
- VIP17/MAL, a lipid raft-associated protein, is involved in apical transport in MDCK cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Raft association of SNAP receptors acting in apical trafficking in Madin-Darby canine kidney cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Annexin XIIIb associates with lipid microdomains to function in apical delivery.The Journal of cell biology · 1998Article
- The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells.The Journal of cell biology · 1998Article
- Identification of two regions in apolipoprotein B100 that are exposed on the cytosolic side of the endoplasmic reticulum membrane.The Journal of cell biology · 1998Article
- Cholesterol is required for surface transport of influenza virus hemagglutinin.The Journal of cell biology · 1998Article
- Rab17 regulates membrane trafficking through apical recycling endosomes in polarized epithelial cells.The Journal of cell biology · 1998Article
- Caveolin-1 and -2 in the exocytic pathway of MDCK cells.The Journal of cell biology · 1998Article
- Na,K-ATPase transport from endoplasmic reticulum to Golgi requires the Golgi spectrin-ankyrin G119 skeleton in Madin Darby canine kidney cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Interaction of influenza virus haemagglutinin with sphingolipid-cholesterol membrane domains via its transmembrane domain.The EMBO journal · 1997Article
- Myosin II is involved in the production of constitutive transport vesicles from the TGN.The Journal of cell biology · 1997Article
- Lysosomes behave as Ca2+-regulated exocytic vesicles in fibroblasts and epithelial cells.The Journal of cell biology · 1997Article
- Signal transduction by the polymeric immunoglobulin receptor suggests a role in regulation of receptor transcytosis.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have reconstituted polarized protein transport in streptolysin O-permeabilized MDCK cells from the TGN to the basolateral surface and to the apical surface. These transport steps are dependent on temperature, energy and exogenously supplied cytosol. Using this in vitro system we show that a whole tail peptide (WT peptide) corresponding to the cytoplasmic tail of a basolaterally sorted protein, the vesicular stomatitis virus glycoprotein (VSV G) inhibits the TGN to basolateral transport but does not affect any other transport step. Inhibition of VSV G transport to basolateral surface by WT peptide did not result in missorting of the protein to the apical surface. Mutation of the single tyrosine residue in the WT peptide reduced its inhibitory potency four- to fivefold. These results suggest that the VSV G tail physically interacts with a component of the sorting machinery. Using a cross-linking approach, we have identified proteins that associate with the cytoplasmic tail domain of VSV G. One of these polypeptides, Tin-2 (Tail interacting protein-2), associates with VSV G in the TGN, the site of protein sorting, but not in the ER nor at the cell surface. Tin-2 does not associate with apically targeted hemagglutinin. WT peptide that inhibited the basolateral transport of VSV G also inhibited the association of Tin-2 with VSV G. Together, these properties make Tin-2 a candidate basolateral sorter. The results demonstrate the usefulness of the SLO-permeabilized cell system in dissecting the sorting machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.