ArticleThe EMBO journal1994
A mutant cysteinyl-tRNA synthetase affecting timing of chromosomal replication initiation in B. subtilis and conferring resistance to a protein kinase C inhibitor.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Pyruvate kinase, a metabolic sensor powering glycolysis, drives the metabolic control of DNA replication.BMC biology · 2022Article
- Multiple links connect central carbon metabolism to DNA replication initiation and elongation in Bacillus subtilis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- A genomic glimpse of aminoacyl-tRNA synthetases in malaria parasite Plasmodium falciparum.BMC genomics · 2009Article
- Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli.The EMBO journal · 2001Article
- Autoregulation of the dnaA-dnaN operon and effects of DnaA protein levels on replication initiation in Bacillus subtilis.Journal of bacteriology · 2001Article
- Limiting DNA replication to once and only once.EMBO reports · 2000Review
- Diversity and specificity of protein-phosphorylating systems in bacteria.Folia microbiologica · 1997Review
- Characterization of cell cycle events during the onset of sporulation in Bacillus subtilis.Journal of bacteriology · 1995Article
- Resistance to trifluoroperazine, a calmodulin inhibitor, maps to the fabD locus in Escherichia coli.Molecular & general genetics : MGG · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A Bacillus subtilis mutant spnA95 was isolated as resistant at 30 degrees C to the protein kinase C (PKC) inhibitor, sphinganine, and temperature sensitive for growth. As deduced by flow cytometry measurements, the mutant has a 35% reduced initiation mass at permissive temperature, resulting in initiation of DNA replication much earlier in the cell cycle than in the wild type. This modification is accompanied by a change in cell size, as determined by phase-contrast microscopy and flow cytometry. Therefore, this strain displays the characteristics of a novel cell clock mutant. spnA is a newly identified gene in B.subtilis and was shown to encode a cysteinyl-tRNA synthetase. At non-permissive temperature, the mutant was defective in the synthesis of P70, a protein with several characteristics of PKC (a cysteine-rich protein). As one possibility, we propose that the altered timing of replication may be due to the reduced synthesis of specific cysteine-rich proteins normally involved in controlling chromosomal replication initiation in B. subtilis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.