ArticleIn vitro cellular & developmental biology. Animal1994
Primary culture of gill epithelial cells from the sea bass Dicentrarchus labrax.
Article in In vitro cellular & developmental biology. Animal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- In vitro protein expression profile of cultivated muscle cells from Labeo rohita.Scientific reports · 2025Article
- In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish.International journal of molecular sciences · 2022Article
- Development and characterization of a new gill cell line from the striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878).Fish physiology and biochemistry · 2022Article
- Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles.Nanomaterials (Basel, Switzerland) · 2021Article
- The Last Half Century of Fish Explant and Organ Culture.Zebrafish · 2021Article
- Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus.Scientific reports · 2020Article
- Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822).Fish physiology and biochemistry · 2020Article
- Establishment and characterization of a caudal fin-derived cell line, AOF, from the Oscar, Astronotus ocellatus.Fish physiology and biochemistry · 2019Article
- Article
- Establishment and characterization of a cell line from the Chinese soft-shelled turtle Pelodiscus sinensis.In vitro cellular & developmental biology. Animal · 2016Article
- A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864).Cytotechnology · 2016Article
- Development and characterization of a cell line WAF from freshwater shark Wallago attu.Molecular biology reports · 2014Article
- Development and characterization of a new cell line CF from caudal fin of knifefish, Chitala chitala (Hamilton-Buchanan).In vitro cellular & developmental biology. Animal · 2013Article
- Cortisol affects tight junction morphology between pavement cells of rainbow trout gills in single-seeded insert culture.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2011Article
- Regulation of Cl- secretion in seawater fish (Dicentrarchus labrax) gill respiratory cells in primary culture.The Journal of physiology · 1999Article
- Isolation, culture and characterization of a primary fibroblast cell line from channel catfish.Cytotechnology · 1998Article
- Making maxillary barbels with a proximal-distal gradient of Wnt signals in matrix-bound mesenchymal cells.Evolution & developmentArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed the first explant technique that allows the in vitro study of gill physiology and biochemistry in marine species. Gill fragments were cultured at 17 degrees C, in atmospheric PCO2, with nutrient medium (Leibovitz L15), pH 7.8, supplemented with 10% fetal bovine serum and adjusted to the osmolarity of fish plasma (350 mOsm/liter). Coating plates with collagen, gelatin, or polylysin did not improve our results. Decrease in osmotic pressure, removal of bovine serum, or its replacement by fish serum inhibited growth from the explants. Approximately 50% of the explants produced cell growth, and after 4 days of culture a monolayer of contiguous cells was formed. This technique is rapid and does not require the use of enzymes. The cells appeared flat and thin with an epitheloid shape. They looked polygonal with a maximum length of 10 to 50 microm. Evidence that they are unique gill cells is the presence of polymorphic surface crenelations (microplicae), prominent Golgi apparatus, tight junctions and desmosomes. Comparison with in vivo tissue showed them to be epithelial cells having differentiated in a homogeneous population of respiratory-like (pavement) cells. They are polarized with their apical surface facing the culture medium. The development of this culture system represents a new tool for cellular approaches to determine precisely the functions and transport mechanism of gill cells.
Indexed as
Identifiers
8193773What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.