ArticleNucleic acids research1994
Retrotransposition of a marked Drosophila line-like I element in cells in culture.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Review
- Genetically tagged TRE5-A retrotransposons reveal high amplification rates and authentic target site preference in the Dictyostelium discoideum genome.Nucleic acids research · 2011Article
- Bioinformatic analysis of Entamoeba histolytica SINE1 elements.BMC genomics · 2010Article
- Genome wide survey, discovery and evolution of repetitive elements in three Entamoeba species.BMC genomics · 2008Article
- New insights on homology-dependent silencing of I factor activity by transgenes containing ORF1 in Drosophila melanogaster.Genetics · 2000Article
- Cosuppression of I transposon activity in Drosophila by I-containing sense and antisense transgenes.Genetics · 1999Article
- A genetically marked I element in Drosophila melanogaster can be mobilized when ORF2 is provided in trans.Genetics · 1998Article
- Defective I elements introduced into Drosophila as transgenes can regulate reactivity and prevent I-R hybrid dysgenesis.Molecular & general genetics : MGG · 1995Article
- A genetically tagged, defective I element can be complemented by actively transposing I factors in the germline of I-R dysgenic females in Drosophila melanogaster.Molecular & general genetics : MGG · 1995Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have marked a Drosophila transposable element--the LINE-like I element--with an intron-containing indicator gene inserted in place of a large deletion in the I element second ORF encompassing the reverse transcriptase domain, and this marked element was placed downstream to a potent actin promoter. An expression vector for the I element ORFs was also constructed, under the same heterologous promoter. The indicator gene contains a lacZ reporter gene the expression of which is conditioned by retrotransposition of the marked element, thus allowing detection of transposition events by testing for either beta-galactosidase expression or occurrence of spliced DNA molecules. The marked I element was introduced into Drosophila melanogaster cells in culture by transfection. Spliced DNA copies of the marked element and specifically stained beta-galactosidase-expressing cells were detected only upon co-transfection with the I expression vector, thus indicating that an ORF2-deleted element can be complemented in trans for transposition. This simple assay for retrotransposition in Drosophila cells in culture provides a tool for the rapid analysis of the mechanism of I transposition in its cis and trans sequence requirements.
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