ArticleNucleic acids research1994
Probing the structure of mouse Ehrlich ascites cell 5.8S, 18S and 28S ribosomal RNA in situ.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
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- In vivo RNA structural probing of uracil and guanine base-pairing by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC).RNA (New York, N.Y.) · 2019Article
- A Fluorescence in Situ Hybridization Method To Quantify mRNA Translation by Visualizing Ribosome-mRNA Interactions in Single Cells.ACS central science · 2017Article
- Genome-wide profiling of mouse RNA secondary structures reveals key features of the mammalian transcriptome.Genome biology · 2014Article
- Cleavage of rRNA ensures translational cessation in sperm at fertilization.Molecular human reproduction · 2011Article
- A novel antisense RNA regulates at transcriptional level the virulence gene icsA of Shigella flexneri.Nucleic acids research · 2010Article
- Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin.Nature chemical biology · 2010Article
- Characterization of the sequence element directing translation reinitiation in RNA of the calicivirus rabbit hemorrhagic disease virus.Journal of virology · 2007Article
- Secondary structure of two regions in expansion segments ES3 and ES6 with the potential of forming a tertiary interaction in eukaryotic 40S ribosomal subunits.RNA (New York, N.Y.) · 2004Article
- Diffusion-based transport of nascent ribosomes in the nucleus.Molecular biology of the cell · 2003Article
- Conformational changes induced in the Saccharomyces cerevisiae GTPase-associated rRNA by ribosomal stalk components and a translocation inhibitor.Nucleic acids research · 2000Article
- Complementarity between the mRNA 5' untranslated region and 18S ribosomal RNA can inhibit translation.RNA (New York, N.Y.) · 2000Article
- rRNA-complementarity in the 5' untranslated region of mRNA specifying the Gtx homeodomain protein: evidence that base- pairing to 18S rRNA affects translational efficiency.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Comparative analyses of the secondary structures of synthetic and intracellular yeast MFA2 mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- rRNA complementarity within mRNAs: a possible basis for mRNA-ribosome interactions and translational control.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Ribotoxic stress response: activation of the stress-activated protein kinase JNK1 by inhibitors of the peptidyl transferase reaction and by sequence-specific RNA damage to the alpha-sarcin/ricin loop in the 28S rRNA.Molecular and cellular biology · 1997Article
- rRNA-like sequences occur in diverse primary transcripts: implications for the control of gene expression.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Divergence towards a dead end? Cleavage of the divergent domains of ribosomal RNA in apoptosis.Experientia · 1996Review
- Fine mapping of 28S rRNA sites specifically cleaved in cells undergoing apoptosis.Molecular and cellular biology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The secondary structure of mouse Ehrlich ascites 18S, 5.8S and 28S ribosomal RNA in situ was investigated by chemical modification using dimethyl sulphate and 1-cyclohexyl-3-(morpholinoethyl) carbodiimide metho-p-toluene sulphonate. These reagents specifically modify unpaired bases in the RNA. The reactive bases were localized by primer extension followed by gel electrophoresis. The three rRNA species were equally accessible for modification i.e. approximately 10% of the nucleotides were reactive. The experimental data support the theoretical secondary structure models proposed for 18S and 5.8/28S rRNA as almost all modified bases were located in putative single-strand regions of the rRNAs or in helical regions that could be expected to undergo dynamic breathing. However, deviations from the suggested models were found in both 18S and 28S rRNA. In 18S rRNA some putative helices in the 5'-domain were extensively modified by the single-strand specific reagents as was one of the suggested helices in domain III of 28S rRNA. Of the four eukaryote specific expansion segments present in mouse Ehrlich ascites cell 28S rRNA, segments I and III were only partly available for modification while segments II and IV showed average to high modification.
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