ArticleMolecular & general genetics : MGG1994
Genetic analysis of SecY: additional export-defective mutations and factors affecting their phenotypes.
Article in Molecular & general genetics : MGG, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Functional mapping and engineering of the Sec translocon unlocked by a cell-free system.Science advances · 2026Article
- Article
- Lateral gate dynamics of the bacterial translocon during cotranslational membrane protein insertion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance.Journal of industrial microbiology & biotechnology · 2016Article
- Secretome analysis defines the major role of SecDF in Staphylococcus aureus virulence.PloS one · 2013Article
- Dynamics of SecY translocons with translocation-defective mutations.Structure (London, England : 1993) · 2010Article
- Assembly and transport mechanism of tripartite drug efflux systems.Biochimica et biophysica acta · 2009Review
- Structure, binding, and activity of Syd, a SecY-interacting protein.The Journal of biological chemistry · 2009Article
- Signal recognition particle-dependent inner membrane targeting of the PulG Pseudopilin component of a type II secretion system.Journal of bacteriology · 2007Article
- SecY alterations that impair membrane protein folding and generate a membrane stress.The Journal of cell biology · 2007Article
- Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Modeling the effects of prl mutations on the Escherichia coli SecY complex.Journal of bacteriology · 2005Article
- Peculiar properties of DsbA in its export across the Escherichia coli cytoplasmic membrane.Journal of bacteriology · 2005Article
- FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon.EMBO reports · 2005Article
- Translation arrest of SecM is essential for the basal and regulated expression of SecA.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Mutational analysis of transmembrane regions 3 and 4 of SecY, a central component of protein translocase.Journal of bacteriology · 2004Article
- Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae.Nucleic acids research · 2004Article
- Importance of transmembrane segments in Escherichia coli SecY.Molecular genetics and genomics : MGG · 2003Article
- Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states.Molecular genetics and genomics : MGG · 2003Article
- A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects.Journal of bacteriology · 2003Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A number of secY mutants of Escherichia coli showing protein export defects were isolated by a combination of localized mutagenesis and secA-lacZ screening. Most of them were cold sensitive and contained single base substitutions in secY leading to amino acid replacements in various parts of the SecY protein, mainly in the cytoplasmic and the transmembrane domains. A temperature-sensitive mutant with an export defect had the same base substitution as secY24, which was characterized previously. Many cold-sensitive secY mutants exhibited rapid responses to temperature lowering but their apparent defects varied at the permissive temperature. Others exhibited delayed responses to the temperature shift. Some secY mutations, including secY39, interfered with protein export when expressed from a multicopy plasmid, even in the presence of wild-type secY on the chromosome. Such "dominant negative" mutations, including secY-d1, which was studied previously, were all located in either cytoplasmic domain 5 or 6, which is consistent with our previous proposal that the C-terminal region of SecY is important for its function as a protein translocator. We also studied the phenotypes of strains in which one of the secY mutations was combined with the components of the secD operon. Overexpression of secD partially suppressed the secY39 mutation, while overexpression of secF exacerbated the export defects of secY122 and secY125 mutations. Overexpression of "yajC", located within the secD operon, suppressed secY-d1. Although yajC itself proved to be dispensable, its disruption impaired the growth of the secY39 mutant at 42 degrees C. These observations suggest that SecY interacts with SecD, SecF, and the product of yajC.
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