ArticleThe Journal of membrane biology1994
Antidiuretic hormone action in A6 cells: effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption.
Article in The Journal of membrane biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 78 citations in OpenAlex.
- Two adjacent phosphorylation sites in the C-terminus of the channel's α-subunit have opposing effects on epithelial sodium channel (ENaC) activity.Pflugers Archiv : European journal of physiology · 2022Article
- Epithelial sodium channel abundance is decreased by an unfolded protein response induced by hyperosmolality.Physiological reports · 2014Article
- Regulated sodium transport in the renal connecting tubule (CNT) via the epithelial sodium channel (ENaC).Pflugers Archiv : European journal of physiology · 2009Review
- Endogenous ATP release inhibits electrogenic Na⁺ absorption and stimulates Cl⁻ secretion in MDCK cells.Purinergic signalling · 2008Article
- Role of vasopressin in rat distal colon function.The Journal of physiology · 2007Article
- Regulation of NaCl transport in the renal collecting duct: lessons from cultured cells.Pflugers Archiv : European journal of physiology · 2006Review
- Vasopressin-stimulated CFTR Cl- currents are increased in the renal collecting duct cells of a mouse model of Liddle's syndrome.The Journal of physiology · 2005Article
- Acute ENaC stimulation by cAMP in a kidney cell line is mediated by exocytic insertion from a recycling channel pool.The Journal of general physiology · 2005Article
- Stimulation of Xenopus P2Y1 receptor activates CFTR in A6 cells.Pflugers Archiv : European journal of physiology · 2004Article
- Mild dehydration, vasopressin and the kidney: animal and human studies.European journal of clinical nutrition · 2003Review
- Extracellular adenine nucleotides regulate Na+/H+ exchanger NHE3 activity in A6-NHE3 transfectants by a cAMP/PKA-dependent mechanism.The Journal of membrane biology · 2002Article
- Molecular aspects of acute inhibition of Na(+)-H(+) exchanger NHE3 by A(2)-adenosine receptor agonists.The Journal of physiology · 2002Article
- Article
- Control of apical membrane chloride permeability in the renal A6 cell line by nucleotides.The Journal of physiology · 1999Article
- Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific.The Journal of physiology · 1999Article
- Regulation of Cl- secretion in seawater fish (Dicentrarchus labrax) gill respiratory cells in primary culture.The Journal of physiology · 1999Article
- Adenosine inhibits the transfected Na+-H+ exchanger NHE3 in Xenopus laevis renal epithelial cells (A6/C1).The Journal of physiology · 1999Article
- Ras pathway activates epithelial Na+ channel and decreases its surface expression in Xenopus oocytes.Molecular biology of the cell · 1998Article
- Cl- transport by cystic fibrosis transmembrane conductance regulator (CFTR) contributes to the inhibition of epithelial Na+ channels (ENaCs) in Xenopus oocytes co-expressing CFTR and ENaC.The Journal of physiology · 1998Article
- Protease modulation of the activity of the epithelial sodium channel expressed in Xenopus oocytes.The Journal of general physiology · 1998Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of antidiuretic hormone on transepithelial Na+ and Cl- transport and its modulation by aldosterone (10(-6) M) was studied in the Xenopus laevis distal nephron cell line A6-C1 by measuring transepithelial electrophysiological parameters and bidirectional anion fluxes. Vasotocin (or vasopressin) induced a biphasic increase in transepithelial short-circuit current (Isc). Early and late effects were potentiated by aldosterone and could be mimicked by forskolin and BrcAMP, implicating cAMP as a mediator. The early increase in Isc (maximum 1-2 min after hormone addition) was resistant to 50 microM amiloride. Electrophysiological experiments with apical ion substitutions or basolateral bumetanide (0.5 mM), as well as flux studies with 125I- or 36Cl-, indicated that this current represented Cl- secretion. The late increase in Isc appeared with a lag of 2-5 min and was maximal after 15-25 min. It corresponded to an increase in Na+ reabsorption, since it was amiloride sensitive. Bidirectional 36Cl- flux measurements in aldosterone-treated monolayers maintained under open-circuit conditions showed that the large vasotocin-induced increase in Cl- permeability led, in these conditions, to a threefold increase of a baseline Cl- reabsorption. This study shows that vasotocin induces in A6-Cl cells both a rapid increase in Cl- permeability and a slower increase in Na+ transport. The Cl- permeability, which leads to Cl- secretion under short-circuit conditions, contributes, under the more physiological open-circuit conditions, to the transport of Na+ by allowing its co-reabsorption with Cl-.
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