ArticleThe Journal of physiology1994
Endogenous Na(+)-K+ (or NH4+)-2Cl- cotransport in Rana oocytes; anomalous effect of external NH4+ on pHi.
Article in The Journal of physiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Beyond CaPflugers Archiv : European journal of physiology · 2021Article
- The Effect of WNK4 on the Na+-Cl- Cotransporter Is Modulated by Intracellular Chloride.Journal of the American Society of Nephrology : JASN · 2015Article
- Revisiting the NaCl cotransporter regulation by with-no-lysine kinases.American journal of physiology. Cell physiology · 2015Review
- Regulation of the renal Na+-Cl- cotransporter by phosphorylation and ubiquitylation.American journal of physiology. Renal physiology · 2012Review
- Sharpey-Schafer lecture: gas channels.Experimental physiology · 2010Article
- Substrate specificity of Rhbg: ammonium and methyl ammonium transport.American journal of physiology. Cell physiology · 2010Article
- Concentration-dependent effects on intracellular and surface pH of exposing Xenopus oocytes to solutions containing NH3/NH4(+).The Journal of membrane biology · 2009Article
- Regulation of NKCC2 by a chloride-sensing mechanism involving the WNK3 and SPAK kinases.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Further investigation of ionic diffusive properties and of NH4+ pathways in Xenopus laevis oocyte cell membrane.Pflugers Archiv : European journal of physiology · 1996Article
- A voltage-dependent and pH-sensitive proton current in Rana esculenta oocytes.The Journal of membrane biology · 1995Article
- Effects of portal hypertension on responsiveness of rat mesenteric artery and aorta.British journal of pharmacology · 1995Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. In Rana oocytes, measurements with chloride-sensitive microelectrodes show that the mean intracellular chloride activity (34.8 +/- 6.3 mM, n = 79) is three times higher than that expected for the passive distribution of chloride ions across the outer membrane (12.4 mM, mean membrane potential -43 +/- 8.8 mV, n = 79). 2. Reuptake of chloride into oocytes depleted by prolonged exposure to chloride-free saline takes place against the electrochemical gradient. 3. Chloride reuptake does not take place in sodium-free solution or in a sodium-substituted potassium-free solution. It is inhibited by bumetanide (10(-5) M) in the bathing medium. 4. The overall stoichiometry of the transport mechanism deduced from simultaneous measurements of intracellular sodium and chloride using ion-selective electrodes is 1Na+:1K+:2Cl-. 5. Ammonium ions substitute for potassium on the cotransporter. 6. In oocytes smaller than 0.9 mm in diameter, exposure to external ammonium causes an alkaline shift in intracellular pH as the NH3 enters and takes up H+ to form NH4+. We propose that chloride-dependent NH4+ transport contributes to the accumulation of NH4+ and causes the 'postexposure' acidification as the intracellular NH4+ releases H+ to form NH3 which is then lost from the cell. 7. In larger oocytes ammonium exposure produces a rapid reduction in pHi which may be explained in part by cotransport-mediated uptake of NH4+. Evidence is also provided for a second chloride-dependent NH4+ transport mechanism and a chloride-independent process.
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